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Journal of Virology, April 2002, p. 4073-4079, Vol. 76, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.8.4073-4079.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Hepatitis C Virus-Like Particle Morphogenesis

Emmanuelle Blanchard,1 Denys Brand,1 Sylvie Trassard,2 Alain Goudeau,1 and Philippe Roingeard1,2*

Laboratoire de Virologie et,1 Laboratoire de Biologie Cellulaire, E3M-EA3250, IFR 82, Faculté de Médecine et Centre Hospitalier Universitaire, Tours, France2

Received 4 October 2001/ Accepted 9 January 2002

Although much is known about the hepatitis C virus (HCV) genome, first cloned in 1989, little is known about HCV structure and assembly due to the lack of an efficient in vitro culture system for HCV. Using a recombinant Semliki forest virus replicon expressing genes encoding HCV structural proteins, we observed for the first time the assembly of these proteins into HCV-like particles in mammalian cells. This system opens up new possibilities for the investigation of viral morphogenesis and virus-host cell interactions.

* Corresponding author. Mailing address: Laboratoire de Biologie Cellulaire, Faculté de Médecine, 2 bis Boulevard Tonnellé, 37032 Tours, France. Phone: (33) 2 47 36 60 71. Fax: (33) 2 47 36 60 90. E-mail: roingeard{at}med.univ-tours.fr.

Journal of Virology, April 2002, p. 4073-4079, Vol. 76, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.8.4073-4079.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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