This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brockman, M. A.
Right arrow Articles by Knipe, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brockman, M. A.
Right arrow Articles by Knipe, D. M.

 Previous Article  |  Next Article 

Journal of Virology, April 2002, p. 3678-3687, Vol. 76, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.8.3678-3687.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Herpes Simplex Virus Vectors Elicit Durable Immune Responses in the Presence of Preexisting Host Immunity

Mark A. Brockman and David M. Knipe*

Department of Microbiology and Molecular Genetics and Committee on Virology, Harvard Medical School, Boston, Massachusetts 02115

Received 25 July 2001/ Accepted 16 January 2002

Herpes simplex virus (HSV) recombinants are being developed as vaccine vectors for the expression of heterologous antigens. There is concern, however, that preexisting HSV immunity may decrease their effectiveness. We have addressed this issue in an animal model. Immunized mice were inoculated with a replication-defective HSV-1 vector that expressed the Escherichia coli ß-galactosidase protein as a model antigen. We assessed vector efficacy by analyzing the immunoglobulin G (IgG) antibody response and cellular proliferative response directed against ß-galactosidase. We report that the ability of the vector to induce antibody or proliferative responses was not diminished by preexisting immunity to HSV. Of further note, the anti-HSV and anti-ß-galactosidase IgG responses following vector administration were extremely durable in both immunized and naive mice. These results indicate that the ability of a replication-defective HSV-derived vaccine vector to elicit long-lived immune responses in mice is not impaired by prior HSV exposure.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: (617) 432-1934. Fax: (617) 432-0223. E-mail: david_knipe{at}hms.harvard.edu.

Journal of Virology, April 2002, p. 3678-3687, Vol. 76, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.8.3678-3687.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Brockman, M. A., Verschoor, A., Zhu, J., Carroll, M. C., Knipe, D. M. (2006). Optimal Long-Term Humoral Responses to Replication-Defective Herpes Simplex Virus Require CD21/CD35 Complement Receptor Expression on Stromal Cells. J. Virol. 80: 7111-7117 [Abstract] [Full Text]  
  • Lauterbach, H., Ried, C., Epstein, A. L., Marconi, P., Brocker, T. (2005). Reduced immune responses after vaccination with a recombinant herpes simplex virus type 1 vector in the presence of antiviral immunity. J. Gen. Virol. 86: 2401-2410 [Abstract] [Full Text]  
  • Redwood, A. J., Messerle, M., Harvey, N. L., Hardy, C. M., Koszinowski, U. H., Lawson, M. A., Shellam, G. R. (2005). Use of a Murine Cytomegalovirus K181-Derived Bacterial Artificial Chromosome as a Vaccine Vector for Immunocontraception. J. Virol. 79: 2998-3008 [Abstract] [Full Text]  
  • Broberg, E. K., Peltoniemi, J., Nygardas, M., Vahlberg, T., Roytta, M., Hukkanen, V. (2004). Spread and Replication of and Immune Response to {gamma}134.5-Negative Herpes Simplex Virus Type 1 Vectors in BALB/c Mice. J. Virol. 78: 13139-13152 [Abstract] [Full Text]  
  • Lauterbach, H., Kerksiek, K. M., Busch, D. H., Berto, E., Bozac, A., Mavromara, P., Manservigi, R., Epstein, A. L., Marconi, P., Brocker, T. (2004). Protection from Bacterial Infection by a Single Vaccination with Replication-Deficient Mutant Herpes Simplex Virus Type 1. J. Virol. 78: 4020-4028 [Abstract] [Full Text]  
  • Kurt-Jones, E. A., Chan, M., Zhou, S., Wang, J., Reed, G., Bronson, R., Arnold, M. M., Knipe, D. M., Finberg, R. W. (2004). Herpes simplex virus 1 interaction with Toll-like receptor 2 contributes to lethal encephalitis. Proc. Natl. Acad. Sci. USA 101: 1315-1320 [Abstract] [Full Text]  
  • Verschoor, A., Brockman, M. A., Gadjeva, M., Knipe, D. M., Carroll, M. C. (2003). Myeloid C3 Determines Induction of Humoral Responses to Peripheral Herpes Simplex Virus Infection. J. Immunol. 171: 5363-5371 [Abstract] [Full Text]  
  • Lucas, M., Tsitoura, E., Montoya, M., Laliotou, B., Aslanoglou, E., Kouvatsis, V., Entwisle, C., Miller, J., Klenerman, P., Hadziyannis, A., Hadziyannis, S., Borrow, P., Mavromara, P. (2003). Characterization of secreted and intracellular forms of a truncated hepatitis C virus E2 protein expressed by a recombinant herpes simplex virus. J. Gen. Virol. 84: 545-554 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»