This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fuller, D. H.
Right arrow Articles by Murphey-Corb, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fuller, D. H.
Right arrow Articles by Murphey-Corb, M.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Medline Plus Health Information
*Immunization

 Previous Article  |  Next Article 

Journal of Virology, April 2002, p. 3309-3317, Vol. 76, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.7.3309-3317.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Induction of Mucosal Protection against Primary, Heterologous Simian Immunodeficiency Virus by a DNA Vaccine

Deborah Heydenburg Fuller,1 Premeela A. Rajakumar,2 Lawrence A. Wilson,3 Anita M. Trichel,2 James T. Fuller,1 Tim Shipley,1 Mary S. Wu,1 Kathleen Weis,1 Charles R. Rinaldo,2 Joel R. Haynes,1 and Michael Murphey-Corb2*

PowderJect Vaccines, Inc., Madison, Wisconsin 53711,1 University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261,2 Tulane Regional Primate Research Center, Covington, Louisiana 704333

Received 7 August 2001/ Accepted 27 September 2001

An effective vaccine against human immunodeficiency virus (HIV) should protect against mucosal transmission of genetically divergent isolates. As a safe alternative to live attenuated vaccines, the immunogenicity and protective efficacy of a DNA vaccine containing simian immunodeficiency virus (SIV) strain 17E-Fr (SIV/17E-Fr) gag-pol-env was analyzed in rhesus macaques. Significant levels of cytotoxic T lymphocytes (CTL), but low to undetectable serum antibody responses, were observed following multiple immunizations. SIV-specific mucosal antibodies and CTL were also detected in rectal washes and gut-associated lymphoid tissues, respectively. Vaccinated and naive control monkeys were challenged intrarectally with SIV strain DeltaB670 (SIV/DeltaB670), a primary isolate whose env is 15% dissimilar to that of the vaccine strain. Four of seven vaccinees were protected from infection as determined by the inability to identify viral RNA or DNA sequences in the peripheral blood and the absence of anamnestic antibody responses postchallenge. This is the first report of mucosal protection against a primary pathogenic, heterologous isolate of SIV by using a commercially viable vaccine approach. These results support further development of a DNA vaccine for protection against HIV.

* Corresponding author. Mailing address: University of Pittsburgh School of Medicine, Department of Molecular Genetics and Biochemistry, Room E1240 Biomedical Science Tower, Pittsburgh, PA 15261. Phone: (412) 648-9462. Fax: (412) 648-1448. E-mail: mcorb{at}pitt.edu.

Journal of Virology, April 2002, p. 3309-3317, Vol. 76, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.7.3309-3317.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rosati, M., Bergamaschi, C., Valentin, A., Kulkarni, V., Jalah, R., Alicea, C., Patel, V., von Gegerfelt, A. S., Montefiori, D. C., Venzon, D. J., Khan, A. S., Draghia-Akli, R., Van Rompay, K. K. A., Felber, B. K., Pavlakis, G. N. (2009). DNA vaccination in rhesus macaques induces potent immune responses and decreases acute and chronic viremia after SIVmac251 challenge. Proc. Natl. Acad. Sci. USA 106: 15831-15836 [Abstract] [Full Text]  
  • Demberg, T., Boyer, J. D., Malkevich, N., Patterson, L. J., Venzon, D., Summers, E. L., Kalisz, I., Kalyanaraman, V. S., Lee, E. M., Weiner, D. B., Robert-Guroff, M. (2008). Sequential Priming with Simian Immunodeficiency Virus (SIV) DNA Vaccines, with or without Encoded Cytokines, and a Replicating Adenovirus-SIV Recombinant Followed by Protein Boosting Does Not Control a Pathogenic SIVmac251 Mucosal Challenge. J. Virol. 82: 10911-10921 [Abstract] [Full Text]  
  • Bonneh-Barkay, D., Bissel, S. J., Wang, G., Fish, K. N., Nicholl, G. C.B., Darko, S. W., Medina-Flores, R., Murphey-Corb, M., Rajakumar, P. A., Nyaundi, J., Mellors, J. W., Bowser, R., Wiley, C. A. (2008). YKL-40, a Marker of Simian Immunodeficiency Virus Encephalitis, Modulates the Biological Activity of Basic Fibroblast Growth Factor. Am. J. Pathol. 173: 130-143 [Abstract] [Full Text]  
  • Bissel, S. J., Wang, G., Bonneh-Barkay, D., Starkey, A., Trichel, A. M., Murphey-Corb, M., Wiley, C. A. (2008). Systemic and Brain Macrophage Infections in Relation to the Development of Simian Immunodeficiency Virus Encephalitis. J. Virol. 82: 5031-5042 [Abstract] [Full Text]  
  • Naito, T., Kaneko, Y., Kozbor, D. (2007). Oral vaccination with modified vaccinia virus Ankara attached covalently to TMPEG-modified cationic liposomes overcomes pre-existing poxvirus immunity from recombinant vaccinia immunization. J. Gen. Virol. 88: 61-70 [Abstract] [Full Text]  
  • Wilson, N. A., Reed, J., Napoe, G. S., Piaskowski, S., Szymanski, A., Furlott, J., Gonzalez, E. J., Yant, L. J., Maness, N. J., May, G. E., Soma, T., Reynolds, M. R., Rakasz, E., Rudersdorf, R., McDermott, A. B., O'Connor, D. H., Friedrich, T. C., Allison, D. B., Patki, A., Picker, L. J., Burton, D. R., Lin, J., Huang, L., Patel, D., Heindecker, G., Fan, J., Citron, M., Horton, M., Wang, F., Liang, X., Shiver, J. W., Casimiro, D. R., Watkins, D. I. (2006). Vaccine-Induced Cellular Immune Responses Reduce Plasma Viral Concentrations after Repeated Low-Dose Challenge with Pathogenic Simian Immunodeficiency Virus SIVmac239.. J. Virol. 80: 5875-5885 [Abstract] [Full Text]  
  • Bissel, S. J., Wang, G., Trichel, A. M., Murphey-Corb, M., Wiley, C. A. (2006). Longitudinal Analysis of Monocyte/Macrophage Infection in Simian Immunodeficiency Virus-Infected, CD8+ T-Cell-Depleted Macaques that Develop Lentiviral Encephalitis. Am. J. Pathol. 168: 1553-1569 [Abstract] [Full Text]  
  • Barratt-Boyes, S. M., Soloff, A. C., Gao, W., Nwanegbo, E., Liu, X., Rajakumar, P. A., Brown, K. N., Robbins, P. D., Murphey-Corb, M., Day, R. D., Gambotto, A. (2006). Broad cellular immunity with robust memory responses to simian immunodeficiency virus following serial vaccination with adenovirus 5- and 35-based vectors. J. Gen. Virol. 87: 139-149 [Abstract] [Full Text]  
  • Ami, Y., Izumi, Y., Matsuo, K., Someya, K., Kanekiyo, M., Horibata, S., Yoshino, N., Sakai, K., Shinohara, K., Matsumoto, S., Yamada, T., Yamazaki, S., Yamamoto, N., Honda, M. (2005). Priming-Boosting Vaccination with Recombinant Mycobacterium bovis Bacillus Calmette-Guerin and a Nonreplicating Vaccinia Virus Recombinant Leads to Long-Lasting and Effective Immunity. J. Virol. 79: 12871-12879 [Abstract] [Full Text]  
  • Otten, G. R., Schaefer, M., Doe, B., Liu, H., Srivastava, I., zur Megede, J., Kazzaz, J., Lian, Y., Singh, M., Ugozzoli, M., Montefiori, D., Lewis, M., Driver, D. A., Dubensky, T., Polo, J. M., Donnelly, J., O'Hagan, D. T., Barnett, S., Ulmer, J. B. (2005). Enhanced Potency of Plasmid DNA Microparticle Human Immunodeficiency Virus Vaccines in Rhesus Macaques by Using a Priming-Boosting Regimen with Recombinant Proteins. J. Virol. 79: 8189-8200 [Abstract] [Full Text]  
  • Ibarrondo, F. J., Anton, P. A., Fuerst, M., Ng, H. L., Wong, J. T., Matud, J., Elliott, J., Shih, R., Hausner, M. A., Price, C., Hultin, L. E., Hultin, P. M., Jamieson, B. D., Yang, O. O. (2005). Parallel Human Immunodeficiency Virus Type 1-Specific CD8+ T-Lymphocyte Responses in Blood and Mucosa during Chronic Infection. J. Virol. 79: 4289-4297 [Abstract] [Full Text]  
  • Yoshino, N., Lu, F. X.-S., Fujihashi, K., Hagiwara, Y., Kataoka, K., Lu, D., Hirst, L., Honda, M., van Ginkel, F. W., Takeda, Y., Miller, C. J., Kiyono, H., McGhee, J. R. (2004). A Novel Adjuvant for Mucosal Immunity to HIV-1 gp120 in Nonhuman Primates. J. Immunol. 173: 6850-6857 [Abstract] [Full Text]  
  • Kang, S.-M., Guo, L., Yao, Q., Skountzou, I., Compans, R. W. (2004). Intranasal Immunization with Inactivated Influenza Virus Enhances Immune Responses to Coadministered Simian-Human Immunodeficiency Virus-Like Particle Antigens. J. Virol. 78: 9624-9632 [Abstract] [Full Text]  
  • Troyer, J. L., Pecon-Slattery, J., Roelke, M. E., Black, L., Packer, C., O'Brien, S. J. (2004). Patterns of Feline Immunodeficiency Virus Multiple Infection and Genome Divergence in a Free-Ranging Population of African Lions. J. Virol. 78: 3777-3791 [Abstract] [Full Text]  
  • Patterson, L. J., Malkevitch, N., Venzon, D., Pinczewski, J., Gomez-Roman, V. R., Wang, L., Kalyanaraman, V. S., Markham, P. D., Robey, F. A., Robert-Guroff, M. (2004). Protection against Mucosal Simian Immunodeficiency Virus SIVmac251 Challenge by Using Replicating Adenovirus-SIV Multigene Vaccine Priming and Subunit Boosting. J. Virol. 78: 2212-2221 [Abstract] [Full Text]  
  • Doria-Rose, N. A., Ohlen, C., Polacino, P., Pierce, C. C., Hensel, M. T., Kuller, L., Mulvania, T., Anderson, D., Greenberg, P. D., Hu, S.-L., Haigwood, N. L. (2003). Multigene DNA Priming-Boosting Vaccines Protect Macaques from Acute CD4+-T-Cell Depletion after Simian-Human Immunodeficiency Virus SHIV89.6P Mucosal Challenge. J. Virol. 77: 11563-11577 [Abstract] [Full Text]  
  • Kang, S.-M., Yao, Q., Guo, L., Compans, R. W. (2003). Mucosal Immunization with Virus-Like Particles of Simian Immunodeficiency Virus Conjugated with Cholera Toxin Subunit B. J. Virol. 77: 9823-9830 [Abstract] [Full Text]  
  • Patterson, L. J., Malkevitch, N., Pinczewski, J., Venzon, D., Lou, Y., Peng, B., Munch, C., Leonard, M., Richardson, E., Aldrich, K., Kalyanaraman, V. S., Pavlakis, G. N., Robert-Guroff, M. (2003). Potent, Persistent Induction and Modulation of Cellular Immune Responses in Rhesus Macaques Primed with Ad5hr-Simian Immunodeficiency Virus (SIV) env/rev, gag, and/or nef Vaccines and Boosted with SIV gp120. J. Virol. 77: 8607-8620 [Abstract] [Full Text]  
  • Otten, G., Schaefer, M., Greer, C., Calderon-Cacia, M., Coit, D., Kazzaz, J., Medina-Selby, A., Selby, M., Singh, M., Ugozzoli, M., zur Megede, J., Barnett, S. W., O'Hagan, D., Donnelly, J., Ulmer, J. (2003). Induction of Broad and Potent Anti-Human Immunodeficiency Virus Immune Responses in Rhesus Macaques by Priming with a DNA Vaccine and Boosting with Protein-Adsorbed Polylactide Coglycolide Microparticles. J. Virol. 77: 6087-6092 [Abstract] [Full Text]  
  • Voss, G., Manson, K., Montefiori, D., Watkins, D. I., Heeney, J., Wyand, M., Cohen, J., Bruck, C. (2002). Prevention of Disease Induced by a Partially Heterologous AIDS Virus in Rhesus Monkeys by Using an Adjuvanted Multicomponent Protein Vaccine. J. Virol. 77: 1049-1058 [Abstract] [Full Text]  
  • Shacklett, B. L., Shaw, K. E. S., Adamson, L. A., Wilkens, D. T., Cox, C. A., Montefiori, D. C., Gardner, M. B., Sonigo, P., Luciw, P. A. (2002). Live, Attenuated Simian Immunodeficiency Virus SIVmac-M4, with Point Mutations in the Env Transmembrane Protein Intracytoplasmic Domain, Provides Partial Protection from Mucosal Challenge with Pathogenic SIVmac251. J. Virol. 76: 11365-11378 [Abstract] [Full Text]  
  • Fuller, C. L., Choi, Y. K., Fallert, B. A., Capuano, S. III, Rajakumar, P., Murphey-Corb, M., Reinhart, T. A. (2002). Restricted SIV Replication in Rhesus Macaque Lung Tissues During the Acute Phase of Infection. Am. J. Pathol. 161: 969-978 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»