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Journal of Virology, April 2002, p. 3257-3266, Vol. 76, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.7.3257-3266.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Interaction between Parvovirus NS2 Protein and Nuclear Export Factor Crm1 Is Important for Viral Egress from the Nucleus of Murine Cells

Cathy L. Miller{dagger}, and David J. Pintel*

School of Medicine, University of Missouri—Columbia, Columbia, Missouri 65212

Received 19 September 2001/ Accepted 7 January 2002

A mutation that disrupts the interaction between the NS2 protein of minute virus of mice and the nuclear export factor Crm1 results in a block to egress of mutant-generated full virions from the nucleus of infected murine cells. These mutants produce wild-type levels of monomer and dimer replicative DNA forms but are impaired in their ability to generate progeny single-stranded DNA in restrictive murine cells in the first round of infection. The NS2-Crm1 interaction mutant can be distinguished phenotypically from an NS2-null mutant and reveals a role for the Crm1-mediated export pathway at a late step in viral infection.

* Corresponding author. Mailing address: Molecular Microbiology and Immunology, M616 Medical Sciences Building, University of Missouri—Columbia School of Medicine, Columbia, MO 65212. Phone: (573) 882-3920. Fax: (573) 882-4287. E-mail: pinteld{at}missouri.edu

{dagger} Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02215.

Journal of Virology, April 2002, p. 3257-3266, Vol. 76, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.7.3257-3266.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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