This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, Y. Y. L.
Right arrow Articles by Hansen, T. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, Y. Y. L.
Right arrow Articles by Hansen, T. H.

 Previous Article  |  Next Article 

Journal of Virology, March 2002, p. 2796-2803, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.2796-2803.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Physical Association of the K3 Protein of Gamma-2 Herpesvirus 68 with Major Histocompatibility Complex Class I Molecules with Impaired Peptide and ß2-Microglobulin Assembly

Y. Y. Lawrence Yu,1 Michael R. Harris,2 Lonnie Lybarger,1 Lisa A. Kimpler,3 Nancy B. Myers,1 Herbert W. Virgin IV,3 and Ted H. Hansen1*

Departments of Genetics,1 Newborn Medicine,2 Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 631103

Received 10 October 2001/ Accepted 18 December 2001

To persist in the presence of an active immune system, viruses encode proteins that decrease expression of major histocompatibility complex class I molecules by using a variety of mechanisms. For example, murine gamma-2 herpesvirus 68 expresses the K3 protein, which causes the rapid turnover of nascent class I molecules. In this report we show that certain mouse class I alleles are more susceptible than others to K3-mediated down regulation. Prior to their rapid degradation, class I molecules in K3-expressing cells exhibit impaired assembly with ß2-microglobulin. Furthermore, K3 is detected predominantly in association with class I molecules lacking assembly with high-affinity peptides, including class I molecules associated with the peptide loading complex TAP/tapasin/calreticulin. The detection of K3 with class I assembly intermediates raises the possibility that molecular chaperones involved in class I assembly are involved in K3-mediated class I regulation.

* Corresponding author. Mailing address: Department of Genetics, Box 8232, Washington University School of Medicine, 4566 Scott Ave., St. Louis, MO 63110. Phone: (314) 362-2716. Fax: (314) 362-4137. E-mail: hansen{at}genetics.wustl.edu.

Journal of Virology, March 2002, p. 2796-2803, Vol. 76, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.6.2796-2803.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Corcoran, K., Wang, X., Lybarger, L. (2009). Adapter-mediated Substrate Selection for Endoplasmic Reticulum-associated Degradation. J. Biol. Chem. 284: 17475-17487 [Abstract] [Full Text]  
  • Horst, D., van Leeuwen, D., Croft, N. P., Garstka, M. A., Hislop, A. D., Kremmer, E., Rickinson, A. B., Wiertz, E. J. H. J., Ressing, M. E. (2009). Specific Targeting of the EBV Lytic Phase Protein BNLF2a to the Transporter Associated with Antigen Processing Results in Impairment of HLA Class I-Restricted Antigen Presentation. J. Immunol. 182: 2313-2324 [Abstract] [Full Text]  
  • Roder, G., Geironson, L., Bressendorff, I., Paulsson, K. (2008). Viral Proteins Interfering with Antigen Presentation Target the Major Histocompatibility Complex Class I Peptide-Loading Complex. J. Virol. 82: 8246-8252 [Full Text]  
  • Hislop, A. D., Ressing, M. E., van Leeuwen, D., Pudney, V. A., Horst, D., Koppers-Lalic, D., Croft, N. P., Neefjes, J. J., Rickinson, A. B., Wiertz, E. J.H.J. (2007). A CD8+ T cell immune evasion protein specific to Epstein-Barr virus and its close relatives in Old World primates. JEM 204: 1863-1873 [Abstract] [Full Text]  
  • Wang, X., Herr, R. A., Chua, W.-J., Lybarger, L., Wiertz, E. J.H.J., Hansen, T. H. (2007). Ubiquitination of serine, threonine, or lysine residues on the cytoplasmic tail can induce ERAD of MHC-I by viral E3 ligase mK3. JCB 177: 613-624 [Abstract] [Full Text]  
  • Ohmura-Hoshino, M., Goto, E., Matsuki, Y., Aoki, M., Mito, M., Uematsu, M., Hotta, H., Ishido, S. (2006). A Novel Family of Membrane-Bound E3 Ubiquitin Ligases. J Biochem 140: 147-154 [Abstract] [Full Text]  
  • Wang, X., Ye, Y., Lencer, W., Hansen, T. H. (2006). The Viral E3 Ubiquitin Ligase mK3 Uses the Derlin/p97 Endoplasmic Reticulum-associated Degradation Pathway to Mediate Down-regulation of Major Histocompatibility Complex Class I Proteins. J. Biol. Chem. 281: 8636-8644 [Abstract] [Full Text]  
  • Chen, M., Tabaczewski, P., Truscott, S. M., Van Kaer, L., Stroynowski, I. (2005). Hepatocytes Express Abundant Surface Class I MHC and Efficiently Use Transporter Associated with Antigen Processing, Tapasin, and Low Molecular Weight Polypeptide Proteasome Subunit Components of Antigen Processing and Presentation Pathway. J. Immunol. 175: 1047-1055 [Abstract] [Full Text]  
  • Huang, S., Gilfillan, S., Cella, M., Miley, M. J., Lantz, O., Lybarger, L., Fremont, D. H., Hansen, T. H. (2005). Evidence for MR1 Antigen Presentation to Mucosal-associated Invariant T Cells. J. Biol. Chem. 280: 21183-21193 [Abstract] [Full Text]  
  • Wang, X., Connors, R., Harris, M. R., Hansen, T. H., Lybarger, L. (2005). Requirements for the Selective Degradation of Endoplasmic Reticulum-Resident Major Histocompatibility Complex Class I Proteins by the Viral Immune Evasion Molecule mK3. J. Virol. 79: 4099-4108 [Abstract] [Full Text]  
  • Braaten, D. C., Sparks-Thissen, R. L., Kreher, S., Speck, S. H., Virgin, H. W. IV (2005). An Optimized CD8+ T-Cell Response Controls Productive and Latent Gammaherpesvirus Infection. J. Virol. 79: 2573-2583 [Abstract] [Full Text]  
  • Wang, X., Lybarger, L., Connors, R., Harris, M. R., Hansen, T. H. (2004). Model for the Interaction of Gammaherpesvirus 68 RING-CH Finger Protein mK3 with Major Histocompatibility Complex Class I and the Peptide-Loading Complex. J. Virol. 78: 8673-8686 [Abstract] [Full Text]  
  • Bartee, E., Mansouri, M., Hovey Nerenberg, B. T., Gouveia, K., Fruh, K. (2004). Downregulation of Major Histocompatibility Complex Class I by Human Ubiquitin Ligases Related to Viral Immune Evasion Proteins. J. Virol. 78: 1109-1120 [Abstract] [Full Text]  
  • Petersen, J. L., Morris, C. R., Solheim, J. C. (2003). Virus Evasion of MHC Class I Molecule Presentation. J. Immunol. 171: 4473-4478 [Full Text]  
  • Rose, T. M., Ryan, J. T., Schultz, E. R., Raden, B. W., Tsai, C.-C. (2003). Analysis of 4.3 Kilobases of Divergent Locus B of Macaque Retroperitoneal Fibromatosis-Associated Herpesvirus Reveals a Close Similarity in Gene Sequence and Genome Organization to Kaposi's Sarcoma-Associated Herpesvirus. J. Virol. 77: 5084-5097 [Abstract] [Full Text]  
  • Flano, E., Kim, I.-J., Woodland, D. L., Blackman, M. A. (2002). {gamma}-Herpesvirus Latency Is Preferentially Maintained in Splenic Germinal Center and Memory B Cells. JEM 196: 1363-1372 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»