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Journal of Virology, March 2002, p. 2460-2468, Vol. 76, No. 5
0022-538X/02/$04.00+0     DOI: 10.1128/jvi.76.5.2460-2468.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Human Cytomegalovirus UL35 Gene Encodes Two Proteins with Different Functions

*** Yingguang Liu1 and Bonita J. Biegalke1,2*

Department of Biomedical Sciences, College of Osteopathic Medicine,1 Molecular and Cellular Biology Program, Ohio University, Athens, Ohio 457012

Received 17 September 2001/ Accepted 4 December 2001

The human cytomegalovirus (HCMV) virion is a complex structure that contains at least 30 proteins, many of which have been identified. We determined that the HCMV UL35 gene encodes two proteins, including a previously unidentified virion protein. A 22-kDa phosphoprotein (ppUL35A) was translated from a 1.2-kb UL35 transcript by 4 h postinfection; a second phosphoprotein of 75 kDa (ppUL35) was translated from a 2.2-kb transcript predominantly late in infection. The 22-kDa protein localized to the nucleus, while the 75-kDa protein localized to the juxtanuclear compartment and was packaged into virion particles. The 22-kDa protein was identical to the COOH-terminal end of the 75-kDa protein but was not found in virions, thus defining the NH2-terminal portion of the 75-kDa protein as essential for packaging. Expression of the 22-kDa protein inhibited activation of the major immediate-early promoter by ppUL82 (pp71), suggesting that the UL35 22-kDa protein may modulate expression of the major immediate-early gene.

* Corresponding author. Mailing address: Department of Biomedical Sciences, 228 Irvine Hall, Ohio University, Athens, OH 45701. Phone: (740) 593-2377. Fax: (740) 597-2778. E-mail: biegalke{at}ohiou.edu.

Journal of Virology, March 2002, p. 2460-2468, Vol. 76, No. 5
0022-538X/02/$04.00+0     DOI: 10.1128/jvi.76.5.2460-2468.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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