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Journal of Virology, February 2002, p. 2019-2023, Vol. 76, No. 4
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.4.2019-2023.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differential Activation of Influenza A Virus Endonuclease Activity Is Dependent on Multiple Sequence Differences between the Virion RNA and cRNA Promoters

Michael B. Leahy, Giuseppe Zecchin, and George G. Brownlee*

Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

Received 5 July 2001/ Accepted 8 November 2001

Influenza virus endonuclease activity was studied in vitro with model virion RNA (vRNA) and cRNA molecules. We show that endonuclease activity can be partially rescued by transplanting vRNA-like promoter features into the model cRNA promoter. This study defines three distinctive features within the vRNA promoter—absent in the cRNA promoter—that are required for endonuclease cleavage.

* Corresponding author. Mailing address: Chemical Pathology Unit, Sir William Dunn School of Pathology, University of Oxford, South Parks Rd., Oxford OX1 3RE, United Kingdom. Phone: 44 (0)1865 275559. Fax: 44 (0) 1865 275556. E-mail: George.Brownlee{at}path.ox.ac.uk.

Journal of Virology, February 2002, p. 2019-2023, Vol. 76, No. 4
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.4.2019-2023.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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