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Journal of Virology, February 2002, p. 1510-1515, Vol. 76, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.3.1510-1515.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of Gene Transfer Efficiencies and Gene Expression Levels Achieved with Equine Infectious Anemia Virus- and Human Immunodeficiency Virus Type 1-Derived Lentivirus Vectors

J. P. O’Rourke,1 G. C. Newbound,1,{dagger} D. B. Kohn,2 J. C. Olsen,3 and B. A. Bunnell1,4*

Children’s Research Institute, Children’s Hospital,1 Division of Research Immunology/Bone Marrow Transplantation, Childrens Hospital, Los Angeles, California 90027,2 Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599,3 Departments of Pediatrics and Molecular Virology, Immunology and Molecular Genetics, The Ohio State University, Columbus, Ohio 432054

Received 21 May 2001/ Accepted 22 October 2001

This report compares gene transfer efficiencies as well as durations and levels of gene expression for human immunodeficiency virus (HIV) and equine infectious anemia virus (EIAV) lentiviral vectors in a variety of human cell types in vitro. EIAV and HIV vectors transduced equivalent numbers of proliferating and G1/S- and G2/M-arrested cells, and both had very low efficiencies of transduction into G0-arrested cells. Analysis of the levels of both the enhanced green fluorescent protein (EGFP) and mRNA demonstrated that the HIV-transduced cells expressed greater levels of EGFP protein and RNA than the EIAV-transduced cells. Measurements of vector-derived EGFP RNA half-lives were fourfold higher with the HIV vector than with the EIAV vector. Long-term culture of EIAV-transduced human cells showed a significant decrease in the number of cells expressing the transgene; however, no corresponding loss was found in EIAV-transduced equine cells. In contrast, only a moderate decrease in the number of transgene-expressing cells was seen with the HIV vectors. Taken together, these results demonstrate that the EIAV vectors transduced human cells with efficiencies similar to those of the HIV vectors. However, our data indicate that transgene expression from EIAV vectors is limited by the instability of vector-derived RNA transcripts and silencing of the EIAV vectors over time.

* Corresponding author. Mailing address: Children’s Research Institute, Room W512, 700 Children’s Dr., Columbus, OH 43205. Phone: (614) 722-2839. Fax: (614) 722-3273. E-mail: bunnellb{at}pediatrics.ohio-state.edu.

{dagger} Present address: Elanco Animal Health, a division of Eli Lilly and Company, Greenfield, IN 46140.

Journal of Virology, February 2002, p. 1510-1515, Vol. 76, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.3.1510-1515.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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