This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Asakura, K.
Right arrow Articles by Ohara, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Asakura, K.
Right arrow Articles by Ohara, Y.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH

 Previous Article  |  Next Article 

Journal of Virology, December 2002, p. 13049-13054, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.13049-13054.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Epitope-Tagged L* Protein of Theiler's Murine Encephalomyelitis Virus Is Expressed in the Central Nervous System in the Acute Phase of Infection

Kunihiko Asakura,1* Harunobu Murayama,2 Toshiki Himeda,1 and Yoshiro Ohara1

Department of Microbiology, Kanazawa Medical University, Ishikawa 920-0293,1 Division of Pathology, Sendai City Hospital, Sendai 984-0075, Japan2

Received 19 June 2002/ Accepted 9 September 2002

TO subgroup strains of Theiler's murine encephalomyelitis virus (TMEV) synthesize L* protein from an alternative initiation codon. We first demonstrated L* expression in the central nervous system (CNS) of TMEV-infected mice during the acute phase of infection by immunoprecipitation and immunoblotting with anti-L* antibody. In addition, we generated mutant viruses which synthesize FLAG or 3xFLAG epitope-tagged L* protein. With a mutant virus expressing 3xFLAG epitope-tagged L*, designated DA/3xFLAGL*, we investigated L* in the CNS in the acute phase of infection. DA/3xFLAGL* did not change the virus tropism in comparison with wild-type virus, and L* was clearly identified in the CNS in both susceptible and resistant strains of mice. Double immunolabeling studies showed that L* is colocalized with TMEV polyprotein and exclusively expressed in neurons.

* Corresponding author. Mailing address: Department of Microbiology, Kanazawa Medical University, 1-1 Uchinada, Ishikawa 920-0293, Japan. Phone: 81-76-286-2211. Fax: 81-76-286-3961. E-mail: kasakura{at}kanazawa-med.ac.jp.

Journal of Virology, December 2002, p. 13049-13054, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.13049-13054.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Asakura, K., Murayama, H., Himeda, T., Ohara, Y. (2007). Expression of L* protein of Theiler's murine encephalomyelitis virus in the chronic phase of infection. J. Gen. Virol. 88: 2268-2274 [Abstract] [Full Text]  
  • Takano-Maruyama, M., Ohara, Y., Asakura, K., Okuwa, T. (2006). Theiler's Murine Encephalomyelitis Virus Leader Protein Amino Acid Residue 57 Regulates Subgroup-Specific Virus Growth on BHK-21 Cells. J. Virol. 80: 12025-12031 [Abstract] [Full Text]  
  • Lin, X., Ma, X., Rodriguez, M., Roos, R. P. (2004). CD4+ T cells are important for clearance of DA strain of TMEV from the central nervous system of SJL/J mice. Int Immunol 16: 1237-1240 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»