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Journal of Virology, December 2002, p. 13039-13048, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.13039-13048.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Mutant of Human Papillomavirus Type 16 E6 Deficient in Binding {alpha}-Helix Partners Displays Reduced Oncogenic Potential In Vivo

Marie Nguyen,1 Shiyu Song,1 Amy Liem,1 Elliot Androphy,2 Yun Liu,2,{dagger} and Paul F. Lambert1*

Department of Oncology, University of Wisconsin, Madison, Wisconsin,1 Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts2

Received 24 May 2002/ Accepted 22 August 2002

Human papillomaviruses (HPVs) are small DNA tumor viruses that are the causative agent of warts and are associated with many anogenital cancers. The viral gene encoding the E6 protein has been found to be involved in HPV oncogenesis. E6 is known to inactivate the cellular tumor suppressor, p53. In addition, E6 has been shown to bind to a variety of other cellular proteins. The focus of this study was to determine what role the interactions of E6 with a subset of cellular proteins which contain a common {alpha}-helical domain in their E6 binding region ({alpha}-helix partners) play in E6-mediated phenotypes. We generated transgenic mice expressing a mutant of E6, E6I128T, which is defective for binding at least a subset of the {alpha}-helix partners, including E6AP, the ubiquitin ligase that mediates E6-dependent degradation of the p53 protein, to determine whether binding of {alpha}-helix partners plays a role in E6-mediated activities in vivo. Unlike mice expressing the wild-type E6 (strain K14E6WT), the mice expressing E6I128T lacked the ability to alter the radiation-induced block to DNA synthesis and promote the formation of benign skin tumors in conjunction with chemical carcinogens. Additionally, they displayed reduced levels of skin hyperplasia, spontaneous skin tumors, and tumor progression activity compared to those of the K14E6WT mice. From these results, we conclude that a domain in E6 that mediates {alpha}-helix partner binding is critical for E6-induced phenotypes in transgenic mice.

* Corresponding author. Mailing address: McArdle Laboratory for Cancer Research, 1400 University Ave., Madison, WI 53706. Phone: (608) 262-8533. Fax: (608) 262-2824. E-mail: lambert{at}oncology.wisc.edu.

{dagger} Present address: Department of Comparative Genomics, GlaxoSmithKline Pharmaceuticals, King of Prussia, PA 19406-0939.

Journal of Virology, December 2002, p. 13039-13048, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.13039-13048.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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