This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Park, J.-W. Articles by Scholthof, H. B. Search for Related Content PubMed PubMed Citation Articles by Park, J.-W. Articles by Scholthof, H. B.

 Previous Article  |  Next Article 

Journal of Virology, December 2002, p. 12747-12757, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.12747-12757.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Tomato Bushy Stunt Virus Genomic RNA Accumulation Is Regulated by Interdependent cis-Acting Elements within the Movement Protein Open Reading Frames

Jong-Won Park, Bénédicte Desvoyes, and Herman B. Scholthof^*

Department of Plant Pathology and Microbiology and Intercollegiate Faculty of Virology, Texas A&M University, College Station, Texas 77843

Received 22 May 2002/ Accepted 11 September 2002

This study on Tomato bushy stunt virus identified and defined three previously unknown regulatory sequences involved in RNA accumulation that are located within the 3'-proximal nested movement protein genes p22 and p19. The first is a 16-nucleotide (nt) element termed III-A that is positioned at the very 3' end of p22 and is essential for RNA accumulation. Approximately 300 nt upstream of III-A resides an 80-nt inhibitory element (IE) that is obstructive to replication only in the absence of a third regulatory element of 30 nt (SUR-III) that is positioned immediately upstream of III-A. Inspection of the nucleotide sequences predicted that III-A and SUR-III can form looped hairpins. A comparison of different tombusviruses showed, in each case, conservation for potential base pairing between the two predicted hairpin-loops. Insertion of a spacer adjacent to the predicted hairpins had no or a minimal effect on RNA accumulation, whereas an insertion in the putative III-A loop abolished genomic RNA multiplication. We conclude that the sequences composing the predicted III-A and SUR-III hairpin-loops are crucial for optimal RNA accumulation and that the inhibitory effect of IE surfaces when the alleged interaction between SUR-III and III-A is disturbed.

---

* Corresponding author. Mailing address: Department of Plant Pathology and Microbiology and Intercollegiate Faculty of Virology, Texas A&M University, 2132 TAMU, College Station, TX 77843. Phone: (979) 862-1495. Fax: (979) 845-6483. E-mail: herscho{at}tamu.edu.

Present address: Department of Disease and Stress Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.

Present address: Centro de Biologia Molecular "Severo Ochoa," Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain.

---

Journal of Virology, December 2002, p. 12747-12757, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.12747-12757.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Panaviene, Z., Panavas, T., Nagy, P. D. (2005). Role of an Internal and Two 3'-Terminal RNA Elements in Assembly of Tombusvirus Replicase. J. Virol. 79: 10608-10618 [Abstract] [Full Text]  
• Tatsuta, M., Mizumoto, H., Kaido, M., Mise, K., Okuno, T. (2005). The Red Clover Necrotic Mosaic Virus RNA2 trans-Activator Is Also a cis-Acting RNA2 Replication Element. J. Virol. 79: 978-986 [Abstract] [Full Text]  
• Panaviene, Z., Panavas, T., Serva, S., Nagy, P. D. (2004). Purification of the Cucumber Necrosis Virus Replicase from Yeast Cells: Role of Coexpressed Viral RNA in Stimulation of Replicase Activity. J. Virol. 78: 8254-8263 [Abstract] [Full Text]