This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Okada, M.
Right arrow Articles by Jeang, K.-T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okada, M.
Right arrow Articles by Jeang, K.-T.

 Previous Article  |  Next Article 

Journal of Virology, December 2002, p. 12564-12573, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.12564-12573.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differential Requirements for Activation of Integrated and Transiently Transfected Human T-Cell Leukemia Virus Type 1 Long Terminal Repeat

Masahiko Okada and Kuan-Teh Jeang*

Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0460

Received 24 January 2002/ Accepted 13 September 2002

Adult T-cell leukemia (ATL) cells contain integrated human T-cell leukemia virus type 1 (HTLV-1) proviruses. Although the exact sequence of events leading to the development of ATL remains incompletely resolved, expression of the integrated HTLV-1 long terminal repeat (LTR) is likely required at some point during the process of T-cell transformation. While much has been learned about the regulated expression of transiently transfected LTR reporter plasmids, an analysis of factors required for expression of chromosomally integrated HTLV-1 LTR has not been done. Here, we have constructed CHOK1 and HeLa cells that contain an integrated HTLV-1 LTR-luciferase gene. Using these cells, we have compared the requirements for activation of transiently transfected versus stably integrated HTLV-1 LTR. We observed different requirements for CREB, p300, and P/CAF in the expression of transiently transfected versus stably integrated HTLV-1 LTR. Furthermore, with dominant-negative mutants of CREB, p300, and P/CAF, we found that activation of integrated HTLV-1 LTR by an ambient stress signal, UV-C, proceeds through a path mechanistically distinct from that used by viral oncoprotein, Tax. Our findings point to additional complexities in the regulated expression of HTLV-1 proviruses compared with those hitherto revealed through transfection studies.

* Corresponding author. Mailing address: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 4, Rm. 306, 9000 Rockville Pike, Bethesda, MD 20892-0460. Phone: (301) 496-6680. Fax: (301) 480-3686. E-mail: kj7e{at}nih.gov.

Journal of Virology, December 2002, p. 12564-12573, Vol. 76, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.24.12564-12573.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Iyer, S. R., Yu, D., Biancotto, A., Margolis, L. B., Wu, Y. (2009). Measurement of Human Immunodeficiency Virus Type 1 Preintegration Transcription by Using Rev-Dependent Rev-CEM Cells Reveals a Sizable Transcribing DNA Population Comparable to That from Proviral Templates. J. Virol. 83: 8662-8673 [Abstract] [Full Text]  
  • Landry, S., Halin, M., Vargas, A., Lemasson, I., Mesnard, J.-M., Barbeau, B. (2009). Upregulation of Human T-Cell Leukemia Virus Type 1 Antisense Transcription by the Viral Tax Protein. J. Virol. 83: 2048-2054 [Abstract] [Full Text]  
  • Clerc, I., Polakowski, N., Andre-Arpin, C., Cook, P., Barbeau, B., Mesnard, J.-M., Lemasson, I. (2008). An Interaction between the Human T Cell Leukemia Virus Type 1 Basic Leucine Zipper Factor (HBZ) and the KIX Domain of p300/CBP Contributes to the Down-regulation of Tax-dependent Viral Transcription by HBZ. J. Biol. Chem. 283: 23903-23913 [Abstract] [Full Text]  
  • Oskarsson, T., Hreggvidsdottir, H. S., Agnarsdottir, G., Matthiasdottir, S., Ogmundsdottir, M. H., Jonsson, S. R., Georgsson, G., Ingvarsson, S., Andresson, O. S., Andresdottir, V. (2007). Duplicated Sequence Motif in the Long Terminal Repeat of Maedi-Visna Virus Extends Cell Tropism and Is Associated with Neurovirulence. J. Virol. 81: 4052-4057 [Abstract] [Full Text]  
  • Lemasson, I., Lewis, M. R., Polakowski, N., Hivin, P., Cavanagh, M.-H., Thebault, S., Barbeau, B., Nyborg, J. K., Mesnard, J.-M. (2007). Human T-Cell Leukemia Virus Type 1 (HTLV-1) bZIP Protein Interacts with the Cellular Transcription Factor CREB To Inhibit HTLV-1 Transcription. J. Virol. 81: 1543-1553 [Abstract] [Full Text]  
  • Zhang, L., Liu, M., Merling, R., Giam, C.-Z. (2006). Versatile Reporter Systems Show that Transactivation by Human T-Cell Leukemia Virus Type 1 Tax Occurs Independently of Chromatin Remodeling Factor BRG1.. J. Virol. 80: 7459-7468 [Abstract] [Full Text]  
  • Moriuchi, M., Moriuchi, H. (2006). Induction of Lactoferrin Gene Expression in Myeloid or Mammary Gland Cells by Human T-Cell Leukemia Virus Type 1 (HTLV-1) Tax: Implications for Milk-Borne Transmission of HTLV-1. J. Virol. 80: 7118-7126 [Abstract] [Full Text]  
  • Lemasson, I., Polakowski, N. J., Laybourn, P. J., Nyborg, J. K. (2006). Tax-dependent Displacement of Nucleosomes during Transcriptional Activation of Human T-Cell Leukemia Virus Type 1. J. Biol. Chem. 281: 13075-13082 [Abstract] [Full Text]  
  • Miyazato, A., Sheleg, S., Iha, H., Li, Y., Jeang, K.-T. (2005). Evidence for NF-{kappa}B- and CBP-Independent Repression of p53's Transcriptional Activity by Human T-Cell Leukemia Virus Type 1 Tax in Mouse Embryo and Primary Human Fibroblasts. J. Virol. 79: 9346-9350 [Abstract] [Full Text]  
  • Matsuoka, M., Jeang, K.-T. (2005). Human T-Cell Leukemia Virus Type I at Age 25: A Progress Report. Cancer Res. 65: 4467-4470 [Abstract] [Full Text]  
  • Tardif, M. R., Tremblay, M. J. (2005). Tetraspanin CD81 Provides a Costimulatory Signal Resulting in Increased Human Immunodeficiency Virus Type 1 Gene Expression in Primary CD4+ T Lymphocytes through NF-{kappa}B, NFAT, and AP-1 Transduction Pathways. J. Virol. 79: 4316-4328 [Abstract] [Full Text]  
  • Moriuchi, M., Moriuchi, H. (2004). Seminal Fluid Enhances Replication of Human T-Cell Leukemia Virus Type 1: Implications for Sexual Transmission. J. Virol. 78: 12709-12711 [Abstract] [Full Text]  
  • Lemasson, I., Polakowski, N. J., Laybourn, P. J., Nyborg, J. K. (2004). Transcription Regulatory Complexes Bind the Human T-Cell Leukemia Virus 5' and 3' Long Terminal Repeats To Control Gene Expression. Mol. Cell. Biol. 24: 6117-6126 [Abstract] [Full Text]  
  • Livengood, J. A., Nyborg, J. K. (2004). The high-affinity Sp1 binding site in the HTLV-1 promoter contributes to Tax-independent basal expression. Nucleic Acids Res 32: 2829-2837 [Abstract] [Full Text]  
  • Dumais, N., Pare, M.-E., Mercier, S., Bounou, S., Marriot, S. J., Barbeau, B., Tremblay, M. J. (2003). T-Cell Receptor/CD28 Engagement When Combined with Prostaglandin E2 Treatment Leads to Potent Activation of Human T-Cell Leukemia Virus Type 1. J. Virol. 77: 11170-11179 [Abstract] [Full Text]  
  • Georges, S. A., Giebler, H. A., Cole, P. A., Luger, K., Laybourn, P. J., Nyborg, J. K. (2003). Tax Recruitment of CBP/p300, via the KIX Domain, Reveals a Potent Requirement for Acetyltransferase Activity That Is Chromatin Dependent and Histone Tail Independent. Mol. Cell. Biol. 23: 3392-3404 [Abstract] [Full Text]  
  • Vidricaire, G., Tardif, M. R., Tremblay, M. J. (2003). The Low Viral Production in Trophoblastic Cells Is Due to a High Endocytic Internalization of the Human Immunodeficiency Virus Type 1 and Can Be Overcome by the Pro-inflammatory Cytokines Tumor Necrosis Factor-alpha and Interleukin-1. J. Biol. Chem. 278: 15832-15841 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»