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Journal of Virology, December 2002, p. 12265-12273, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12265-12273.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Saccharomyces cerevisiae Is Permissive for Replication of Bovine Papillomavirus Type 1

Kong-Nan Zhao* and Ian H. Frazer

Centre for Immunology and Cancer Research, The University of Queensland, Princess Alexandra Hospital, Brisbane, Queensland 4102, Australia

Received 10 June 2002/ Accepted 19 July 2002

We recently demonstrated that Saccharomyces cerevisiae protoplasts can take up bovine papillomavirus type 1 (BPV1) virions and that viral episomal DNA is replicated after uptake. Here we demonstrate that BPV virus-like particles are assembled in infected S. cerevisiae cultures from newly synthesized capsid proteins and also package newly synthesized DNA, including full-length and truncated viral DNA and S. cerevisiae-derived DNA. Virus particles prepared in S. cerevisiae are able to convey packaged DNA to Cos1 cells and to transform C127 cells. Infectivity was blocked by antisera to BPV1 L1 but not antisera to BPV1 E4. We conclude that S. cerevisiae is permissive for the replication of BPV1 virus.

* Corresponding author. Mailing address: Centre for Immunology and Cancer Research, The University of Queensland, Princess Alexandra Hospital, Brisbane, QLD 4102, Australia. Phone: 61-7-3240-5912. Fax: 61-7-3240-5946. E-mail: knzhao{at}cicr.uq.edu.au.

Journal of Virology, December 2002, p. 12265-12273, Vol. 76, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.23.12265-12273.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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