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Journal of Virology, November 2002, p. 11721-11728, Vol. 76, No. 22
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.22.11721-11728.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Unique Characteristics of a Picornavirus Internal Ribosome Entry Site from the Porcine Teschovirus-1 Talfan

Yoshihiro Kaku,1 Louisa S. Chard,2 Toru Inoue,3 and Graham J. Belsham2*

Department of Infectious Diseases, National Institute of Animal Health, Tsukuba, Ibaraki 305-0856,1 Department of Exotic Diseases, National Institute of Animal Health, Kodaira, Tokyo 187-0022, Japan,3 BBSRC Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, United Kingdom2

Received 3 June 2002/ Accepted 5 August 2002

The teschoviruses constitute a recently defined picornavirus genus. Most of the genome sequence of the porcine teschovirus-1 (PTV) Talfan and several other strains is known. We now demonstrate that initiation of protein synthesis occurs at nucleotide (nt) 412 on the PTV Talfan RNA and that nt 1 to 405 contains an internal ribosome entry site (IRES) that functions efficiently in vitro and within mammalian cells. In comparison with other picornavirus IRES elements, the PTV IRES is relatively short and lacks a significant polypyrimidine tract near the 3' end. Expression of an enterovirus 2A protease, which induces cleavage of eIF4G within the translation initiation complex eIF4F, has little effect on the PTV IRES activity within BHK cells. The PTV IRES has a unique set of properties and represents a new class of picornavirus IRES element.

* Corresponding author. Mailing address: BBSRC Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, United Kingdom. Phone: 44 1483 232441. Fax: 44 1483 232448. E-mail: graham.belsham{at}bbsrc.ac.uk.

Journal of Virology, November 2002, p. 11721-11728, Vol. 76, No. 22
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.22.11721-11728.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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Copyright © 2002 by the American Society for Microbiology. All rights reserved.