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Journal of Virology, November 2002, p. 11143-11147, Vol. 76, No. 21
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.21.11143-11147.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of the Hepatitis C Virus E2 Glycoprotein Binding Site on the Large Extracellular Loop of CD81

Heidi E. Drummer,* Kirilee A. Wilson, and Pantelis Poumbourios

St. Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia

Received 22 April 2002/ Accepted 23 July 2002

The binding of hepatitis C virus glycoprotein E2 to the large extracellular loop (LEL) of CD81 has been shown to modulate human T-cell and NK cell activity in vitro. Using random mutagenesis of a chimera of maltose-binding protein and LEL residues 113 to 201, we have determined that the E2-binding site on CD81 comprises residues Ile182, Phe186, Asn184, and Leu162. These findings reveal an E2-binding surface of approximately 806 Å2 and potential target sites for the development of small-molecule inhibitors of E2 binding.

* Corresponding author. Mailing address: St. Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia. Phone: 61 3 9288 2480. Fax: 61 3 9416 2676. E-mail: heidid{at}ariel.its.unimelb.edu.au.

Journal of Virology, November 2002, p. 11143-11147, Vol. 76, No. 21
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.21.11143-11147.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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