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Journal of Virology, November 2002, p. 11133-11138, Vol. 76, No. 21
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.21.11133-11138.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Implication of the Lymphocyte-Specific Nuclear Body Protein Sp140 in an Innate Response to Human Immunodeficiency Virus Type 1

Navid Madani,1,{dagger} Robert Millette,1 Emily J. Platt,1 Mariana Marin,1 Susan L. Kozak,1 Donald B. Bloch,2 and David Kabat1*

Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon 97201-3098,1 Department of Medicine, Massachusetts General Hospital, Charlestown, Massachusetts 021292

Received 5 April 2002/ Accepted 18 July 2002

The viral infectivity factor (Vif) of human immunodeficiency virus type 1 (HIV-1) neutralizes an unidentified antiviral pathway that occurs only in nonpermissive (NP) cells. Using a yeast two-hybrid screen of a human lymphocyte cDNA library, we identified several potential Vif partners. One, the nuclear body protein Sp140, was found specifically in all NP cells (n = 12 cell lines tested; P <= 0.001), and HIV-1 infection induced its partial dispersal from nuclear bodies into cytosolic colocalization with Vif. Our results implicate Sp140 in a response to HIV-1 that may be related to or coordinated with the pathway that inactivates HIV-1 lacking vif.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97201-3098. Phone: (503) 494-8442. Fax: (503) 494-8393. E-mail: kabat{at}ohsu.edu.

{dagger} Present address: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.

Journal of Virology, November 2002, p. 11133-11138, Vol. 76, No. 21
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.21.11133-11138.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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