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Journal of Virology, November 2002, p. 10882-10893, Vol. 76, No. 21
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.21.10882-10893.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Biology of Ovine Adenovirus Infection of Nonpermissive Cells

Daniel Kümin,1 Christian Hofmann,1 Michael Rudolph,2 Gerald W. Both,3 and Peter Löser1*

DeveloGen AG, Niederlassung Berlin, 13125 Berlin,1 Institut für Zoo- und Wildtierforschung, 10315 Berlin, Germany,2 Molecular Science, CSIRO, North Ryde, New South Wales 2113, Australia3

Received 7 May 2002/ Accepted 24 July 2002

Nonhuman adenoviruses, including those of the genus Atadenovirus, have the potential to serve as vectors for vaccine and gene therapy applications in humans, since they are resistant to preexisting immunity induced by human adenoviruses in the majority of the population. In this study, we elucidate the outcome of infection by ovine adenovirus type 7 isolate 287 (OAdV) of several nonovine cell types. We show here that OAdV infects a wide range of nonovine cells but is unable to complete its replication cycle in any of them. In nonovine, nonfibroblast cells, viral replication is blocked at an early stage before the onset of, or early in, DNA replication. Some fibroblasts, on the other hand, allow viral DNA replication but block virus production at a later stage during or after the translation of late viral proteins. Late viral proteins are expressed in cells where viral DNA replication takes place, albeit at a reduced level. Significantly, late proteins are not properly processed, and their cellular distribution differs from that observed in infected ovine cells. Thus, our results clearly show that OAdV infection of all nonovine cells tested is abortive even if significant viral DNA replication occurs. These findings have significant positive implications with respect to the safety of the vector system and its future use in humans.

* Corresponding author. Mailing address: DeveloGen AG, Niederlassung Berlin, Robert-Rössle-Strasse 10, 13125 Berlin, Germany. Phone: 49 30 94 89 22 88. Fax: 49 30 94 89 29 13. E-mail: ploeser{at}develogen.com.

Journal of Virology, November 2002, p. 10882-10893, Vol. 76, No. 21
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.21.10882-10893.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Loser, P., Hofmann, C., Both, G. W., Uckert, W., Hillgenberg, M. (2003). Construction, Rescue, and Characterization of Vectors Derived from Ovine Atadenovirus. J. Virol. 77: 11941-11951 [Abstract] [Full Text]  


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