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Journal of Virology, October 2002, p. 10540-10545, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10540-10545.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Protective Antiviral Immune Responses to Pseudorabies Virus Induced by DNA Vaccination Using Dimethyldioctadecylammonium Bromide as an Adjuvant

Eugene M. A. van Rooij,1* Harrie L. Glansbeek,2 Luuk A. T. Hilgers,3 Eddie G. te Lintelo,2 Yolanda E. de Visser,1 Wim J. A. Boersma,4 Bart L. Haagmans,5 and Andre T. J. Bianchi1

Central Institute for Animal Disease Control, CIDC-Lelystad,1 Institute for Animal Science and Health, ID-Lelystad, Lelystad,4 Veterinary Faculty, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht,2 CoVaccine B.V., Lelystad,3 Institute of Virology, Erasmus University Rotterdam, Rotterdam, The Netherlands5

Received 6 March 2002/ Accepted 9 July 2002

To enhance the efficacy of a DNA vaccine against pseudorabies virus (PRV), we evaluated the adjuvant properties of plasmids coding for gamma interferon or interleukin-12, of CpG immunostimulatory motifs, and of the conventional adjuvants dimethyldioctadecylammonium bromide in water (DDA) and sulfolipo-cyclodextrin in squalene in water. We demonstrate that a DNA vaccine combined with DDA, but not with the other adjuvants, induced significantly stronger immune responses than plasmid vaccination alone. Moreover, pigs vaccinated in the presence of DDA were protected against clinical disease and shed significantly less PRV after challenge infection. This is the first study to demonstrate that DDA, a conventional adjuvant, enhances DNA vaccine-induced antiviral immunity.

* Corresponding author. Mailing address: Central Institute for Animal Disease Control, CIDC-Lelystad, P.O. Box 2004, Houtribweg 39, Lelystad NL8203 AA, The Netherlands. Phone: 31-320-238238. Fax: 31-320-238668. E-mail: e.m.a.vanrooij{at}id.wag-ur.nl.

Journal of Virology, October 2002, p. 10540-10545, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10540-10545.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dory, D., Torche, A.-M., Beven, V., Cariolet, R., Jestin, A. (2007). Limited Protection Conferred by a DNA Vaccine against a Lethal Pseudorabies Virus Infection at Day 5 Postvaccination. CVI 14: 470-473 [Abstract] [Full Text]  


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