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Journal of Virology, October 2002, p. 10264-10269, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10264-10269.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mimovirus: a Novel Form of Vaccine That Induces Hepatitis B Virus-Specific Cytotoxic T-Lymphocyte Responses In Vivo

Yu-Zhang Wu, Jian-Ping Zhao,* Ying Wan, Zheng-Cai Jia, Wei Zhou, Jiang Bian, Bing Ni, Li-Yun Zou, and Yan Tang

Institute of Immunology, Third Military Medical University, District Shapingba, Chongqing 400038, People's Republic of China

Received 12 April 2002/ Accepted 9 July 2002

CD8+ cytotoxic T lymphocytes (CTLs) are now recognized as important mediators of immunity against intracellular pathogens, including human immunodeficiency virus and tumors. How to efficiently evoke antigen-specific CTL responses in vivo has become a crucial problem in the development of modern vaccines. Here, we developed a completely novel CTL vaccine—mimovirus, which is a kind of virus-size particulate antigen delivery system. It was formed by the self-assembly of a cationic peptide containing 18 lysines and a CTL-epitope peptide of HBsAg28-39, with a plasmid encoding mouse interleukin-12 (IL-12) through electrostatic interactions. We examined the formation of mimovirus by DNA retardation assay, DNase I protection assay, and transmission electron microscopy and demonstrated that mimovirus could efficiently transfer the plasmid encoding IL-12 into mammalian cells such as P815 cells in vitro. Furthermore, it was proved that mimovirus could induce an HBsAg28-39-specific CTL response in vivo. Considering its effectiveness, flexibility, and defined composition, mimovirus is potentially a novel system for vaccination against intracellular pathogens and tumors.

* Corresponding author. Mailing address: Institute of Immunology, Third Military Medical University, Ave. Gaotanyan 30th, District Shapingba, Chongqing 400038, People's Republic of China. Phone: 86-23-68752236. Fax: 86-23-68752789. E-mail: zhaojpim{at}yahoo.com.

Journal of Virology, October 2002, p. 10264-10269, Vol. 76, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.20.10264-10269.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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