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Journal of Virology, January 2002, p. 905-911, Vol. 76, No. 2
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.2.905-911.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Intracellular Distribution of Human T-Cell Leukemia Virus Type 1 Gag Proteins Is Independent of Interaction with Intracellular Membranes

Isabelle Le Blanc,1* Vincent Blot,1 Isabelle Bouchaert,2 Jean Salamero,3 Bruno Goud,3 Arielle R. Rosenberg,1 and Marie-Christine Dokhélar1

INSERM U332,1 Service de Cytofluorométrie, ICGM,2 CNRS UMR 144, Institut Curie, Paris, France3

Received 25 April 2001/ Accepted 27 October 2001

Retrovirus Gag proteins are synthesized on free ribosomes, and are sufficient to govern the assembly and release of virus particles. Like type C retroviruses, human T-cell leukemia virus type 1 (HTLV-1) assembles and buds at the plasma membrane. After immunofluorescence staining, HTLV-1 Gag proteins appear as punctuated intracellular clusters, which suggests that they are associated either with intracellular membranes or with the plasma membrane. However, colocalization experiments using a panel of markers demonstrated that Gag proteins were not associated with the membranes involved in the secretory or endocytosis pathway. Small amounts of Gag proteins were detected at the plasma membrane and colocalized with the envelope glycoproteins. Moreover, Gag proteins were excluded from streptolysin-O permeabilized cells and in this respect behaved like cytoplasmic proteins. This suggests that the trafficking of HTLV-1 Gag proteins through the cytoplasm of the host cell is independent of any cell membrane system.

* Corresponding author. Mailing address: Department of Biochemistry, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva 4, Switzerland. Phone: 41 22 702 64 82. Fax: 41 22 702 64 64. E-mail: Isabelle.LeBlanc{at}biochem.unige.ch.

Journal of Virology, January 2002, p. 905-911, Vol. 76, No. 2
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.2.905-911.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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