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Journal of Virology, October 2002, p. 10024-10029, Vol. 76, No. 19
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.19.10024-10029.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The PPPY Motif of Human T-Cell Leukemia Virus Type 1 Gag Protein Is Required Early in the Budding Process

Isabelle Le Blanc,1,{dagger} Marie-Christine Prévost,2 Marie-Christine Dokhélar,1 and Arielle R. Rosenberg1*

INSERM U332, Institut Cochin de Génétique Moléculaire,1 Unité d'Oncologie Virale, Institut Pasteur, Paris, France2

Received 6 December 2001/ Accepted 30 June 2002

Domains required late in the virus budding process (L domains) have been identified in the Gag proteins of a number of retroviruses. Here we show that the human T-cell leukemia virus type 1 candidate L domain motif PPPY is indeed required for virus production. Strikingly, however, mutation of this motif arrested virus particles at an earlier stage in the budding process than was seen for mutation of the L domain motifs thus far described for retroviruses. In view of the exchangeability of such domains, we propose that the retrovirus budding process may involve a continuum from bud formation to membrane fission.

* Corresponding author. Present address: Équipe Virus de l'Hépatite C, Institut Cochin, 22, rue Méchain, 75014 Paris, France. Phone: 33 1 40 51 64 93. Fax: 33 1 40 51 64 07. E-mail: arielle{at}cochin.inserm.fr.

{dagger} Present address: Department of Biochemistry, University of Geneva, 31211 Geneva 4, Switzerland.

Journal of Virology, October 2002, p. 10024-10029, Vol. 76, No. 19
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.19.10024-10029.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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