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Journal of Virology, September 2002, p. 8966-8972, Vol. 76, No. 17
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.17.8966-8972.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genetically Stable Picornavirus Expression Vectors with Recombinant Internal Ribosomal Entry Sites

Andrew T. Dufresne, Elena Y. Dobrikova, Stacie Schmidt, and Matthias Gromeier*

Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710

Received 26 February 2002/ Accepted 23 May 2002

In many respects, picornaviruses are well suited for their proposed use as immunization vectors. However, their inherent genetic instability hinders application for prophylactic purposes. We demonstrate the improved expression and stability of a heterologous insert through a novel vector design strategy that partially replaces noncoding regulatory sequences with coding sequences for foreign gene products.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710. Phone: (919) 668-6206. Fax: (919) 684-8735. E-mail: grome001{at}mc.duke.edu.

Journal of Virology, September 2002, p. 8966-8972, Vol. 76, No. 17
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.17.8966-8972.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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