This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Malaspina, A.
Right arrow Articles by Fauci, A. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Malaspina, A.
Right arrow Articles by Fauci, A. S.

 Previous Article  |  Next Article 

Journal of Virology, September 2002, p. 8855-8863, Vol. 76, No. 17
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.17.8855-8863.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Human Immunodeficiency Virus Type 1 Bound to B Cells: Relationship to Virus Replicating in CD4+ T Cells and Circulating in Plasma

Angela Malaspina,1 Susan Moir,1* David C. Nickle,2 Eileen T. Donoghue,1 Kisani M. Ogwaro,1 Linda A. Ehler,1 Shuying Liu,1 Jo Ann M. Mican,1 Mark Dybul,1 Tae-Wook Chun,1 James I. Mullins,2,3 and Anthony S. Fauci1

Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892,1 Departments of Microbiology,2 Medicine, University of Washington, Seattle, Washington 981953

Received 5 February 2002/ Accepted 20 May 2002

Human immunodeficiency virus type 1 (HIV-1) virions bind to B cells in the peripheral blood and lymph nodes through interactions between CD21 on B cells and complement-complexed virions. B-cell-bound virions have been shown to be highly infectious, suggesting a unique mode of HIV-1 dissemination by B cells circulating between peripheral blood and lymphoid tissues. In order to investigate the relationship between B-cell-bound HIV-1 and viruses found in CD4+ T cells and in plasma, we examined the genetic relationships of HIV-1 found in the blood and lymph nodes of chronically infected patients with heteroduplex mobility and tracking assays and DNA sequence analysis. In samples from 13 of 15 patients examined, HIV-1 variants in peripheral blood-derived B cells were closely related to virus in CD4+ T cells and more divergent from virus in plasma. In samples from five chronically viremic patients for whom analyses were extended to include lymph node-derived HIV-1 isolates, B-cell-associated HIV-1 and CD4+-T-cell-associated HIV-1 in the lymph nodes were equivalent in their divergence from virus in peripheral blood-derived B cells and generally more distantly related to virus in peripheral blood-derived CD4+ T cells. These results indicates virologic cross talk between B cells and CD4+ T cells within the microenvironment of lymphoid tissues and, to a lesser extent, between cells in lymph nodes and the peripheral blood. These findings also indicate that most of the virus in plasma originates from cells other than CD4+ T cells in the peripheral blood and lymph nodes.

* Corresponding author. Mailing address: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 10 Center Dr., MSC-1576, Building 10, Room 6A02, Bethesda, MD 20892. Phone: (301) 402-4559. Fax: (301) 402-5920. E-mail: smoir{at}niaid.nih.gov.

Journal of Virology, September 2002, p. 8855-8863, Vol. 76, No. 17
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.17.8855-8863.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ho, J., Moir, S., Kulik, L., Malaspina, A., Donoghue, E. T., Miller, N. J., Wang, W., Chun, T.-W., Fauci, A. S., Holers, V. M. (2007). Role for CD21 in the Establishment of an Extracellular HIV Reservoir in Lymphoid Tissues. J. Immunol. 178: 6968-6974 [Abstract] [Full Text]  
  • Sullivan, S. T., Mandava, U., Evans-Strickfaden, T., Lennox, J. L., Ellerbrock, T. V., Hart, C. E. (2005). Diversity, Divergence, and Evolution of Cell-Free Human Immunodeficiency Virus Type 1 in Vaginal Secretions and Blood of Chronically Infected Women: Associations with Immune Status. J. Virol. 79: 9799-9809 [Abstract] [Full Text]  
  • Hira, S. K. (2003). Occurrence and Management of Opportunistic Infections Associated with HIV/AIDS in Asia. Journal of Health Management 5: 237-260 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»