This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Subramanian, C.
Right arrow Articles by Robertson, E. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Subramanian, C.
Right arrow Articles by Robertson, E. S.

 Previous Article  |  Next Article 

Journal of Virology, September 2002, p. 8702-8709, Vol. 76, No. 17
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.17.8702-8709.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Metastatic Suppressor Nm23-H1 Interacts with EBNA3C at Sequences Located between the Glutamine- and Proline-Rich Domains and Can Cooperate in Activation of Transcription

Chitra Subramanian and Erle S. Robertson*

Department of Microbiology and Immunology and the Comprehensive Cancer and Geriatrics Center, University of Michigan Medical School, Ann Arbor, Michigan 48109-0934

Received 21 March 2002/ Accepted 6 June 2002

Epstein-Barr virus (EBV) is a lymphotrophic herpesvirus infecting most of the world's population. It is associated with a number of human lymphoid and epithelial tumors and lymphoproliferative diseases in immunocompromised patients. Recent studies have shown an in vitro and in vivo interaction between the EBV nuclear antigen 3C (EBNA3C) and the metastatic suppressor Nm23-H1, known to be downregulated in human invasive breast carcinoma. In this study, we have identified the domain of EBNA3C that specifically binds to Nm23-H1. This domain lies within the region comprising amino acids 637 to 675 of EBNA3C flanked by the proline- and glutamine-rich domains. Furthermore, we show that Nm23-H1 activates transcription when fused to the Gal4 DNA-binding domain and is coexpressed with a luciferase reporter construct containing the Gal4 binding sites upstream of a basal promoter. Gal4-Nm23-H1, when tethered to the promoter by binding to the Gal4 DNA binding sequences, consistently activated transcription. The level of activation increased when increasing amounts of Gal4-Nm23-H1 were introduced into the system. Moreover, EBNA3C when cotransfected with Gal4-Nm23-H1 enhanced the transcriptional activity. These results suggest that Nm23-H1 may have intrinsic transcription activities in EBV-infected cells and that this activity can be modulated in the presence of the essential latent antigen EBNA3C.

* Corresponding author. Mailing address: Department of Microbiology and Immunology and the Comprehensive Cancer and Geriatrics Center, 3217 CCGC, University of Michigan Medical School, Ann Arbor, MI 48109-0934. Phone: (734) 647-7296. Fax: (734) 647-7296. E-mail: esrobert{at}umich.edu.

Journal of Virology, September 2002, p. 8702-8709, Vol. 76, No. 17
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.17.8702-8709.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kaul, R., Murakami, M., Lan, K., Choudhuri, T., Robertson, E. S. (2009). EBNA3C Can Modulate the Activities of the Transcription Factor Necdin in Association with Metastasis Suppressor Protein Nm23-H1. J. Virol. 83: 4871-4883 [Abstract] [Full Text]  
  • Maruo, S., Wu, Y., Ito, T., Kanda, T., Kieff, E. D., Takada, K. (2009). Epstein-Barr virus nuclear protein EBNA3C residues critical for maintaining lymphoblastoid cell growth. Proc. Natl. Acad. Sci. USA 106: 4419-4424 [Abstract] [Full Text]  
  • Steeg, P. S., Horak, C. E., Miller, K. D. (2008). Clinical-Translational Approaches to the Nm23-H1 Metastasis Suppressor. Clin. Cancer Res. 14: 5006-5012 [Abstract] [Full Text]  
  • Jiang, J., Wei, Y., Shen, J., Liu, D., Chen, X., Zhou, J., Zong, H., Yun, X., Kong, X., Zhang, S., Yang, Y., Gu, J. (2007). Functional Interaction of E1AF and Sp1 in Glioma Invasion. Mol. Cell. Biol. 27: 8770-8782 [Abstract] [Full Text]  
  • Kaul, R., Murakami, M., Choudhuri, T., Robertson, E. S. (2007). Epstein-Barr Virus Latent Nuclear Antigens Can Induce Metastasis in a Nude Mouse Model. J. Virol. 81: 10352-10361 [Abstract] [Full Text]  
  • Kaul, R., Verma, S. C., Murakami, M., Lan, K., Choudhuri, T., Robertson, E. S. (2006). Epstein-Barr Virus Protein Can Upregulate Cyclo-Oxygenase-2 Expression through Association with the Suppressor of Metastasis Nm23-H1. J. Virol. 80: 1321-1331 [Abstract] [Full Text]  
  • Kuppers, D. A., Lan, K., Knight, J. S., Robertson, E. S. (2005). Regulation of Matrix Metalloproteinase 9 Expression by Epstein-Barr Virus Nuclear Antigen 3C and the Suppressor of Metastasis Nm23-H1. J. Virol. 79: 9714-9724 [Abstract] [Full Text]  
  • Murakami, M., Lan, K., Subramanian, C., Robertson, E. S. (2005). Epstein-Barr Virus Nuclear Antigen 1 Interacts with Nm23-H1 in Lymphoblastoid Cell Lines and Inhibits Its Ability To Suppress Cell Migration. J. Virol. 79: 1559-1568 [Abstract] [Full Text]  
  • Verma, S. C., Borah, S., Robertson, E. S. (2004). Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus Up-Regulates Transcription of Human Telomerase Reverse Transcriptase Promoter through Interaction with Transcription Factor Sp1. J. Virol. 78: 10348-10359 [Abstract] [Full Text]  
  • Knight, J. S., Robertson, E. S. (2004). Epstein-Barr Virus Nuclear Antigen 3C Regulates Cyclin A/p27 Complexes and Enhances Cyclin A-Dependent Kinase Activity. J. Virol. 78: 1981-1991 [Abstract] [Full Text]  
  • Rosendorff, A., Illanes, D., David, G., Lin, J., Kieff, E., Johannsen, E. (2004). EBNA3C Coactivation with EBNA2 Requires a SUMO Homology Domain. J. Virol. 78: 367-377 [Abstract] [Full Text]  
  • Krauer, K., Buck, M., Flanagan, J., Belzer, D., Sculley, T. (2004). Identification of the nuclear localization signals within the Epstein-Barr virus EBNA-6 protein. J. Gen. Virol. 85: 165-172 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»