This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weber, F.
Right arrow Articles by Elliott, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weber, F.
Right arrow Articles by Elliott, R. M.

 Previous Article  |  Next Article 

Journal of Virology, August 2002, p. 7949-7955, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.7949-7955.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Bunyamwera Bunyavirus Nonstructural Protein NSs Counteracts the Induction of Alpha/Beta Interferon

Friedemann Weber,1 Anne Bridgen,2 John K. Fazakerley,3 Hein Streitenfeld,1 Nina Kessler,1 Richard E. Randall,4 and Richard M. Elliott2*

Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, D-79008 Freiburg, Germany, and,1 Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 5JR,2 Laboratory for Clinical and Molecular Virology, University of Edinburgh, Edinburgh EH9 1QH,3 School of Biology Sciences, University of St. Andrews, Fife KY16 9TS, Scotland, United Kingdom4

Received 12 February 2002/ Accepted 9 May 2002

Production of alpha/beta interferons (IFN-{alpha}/ß) in response to viral infection is one of the main defense mechanisms of the innate immune system. Many viruses therefore encode factors that subvert the IFN system to enhance their virulence. Bunyamwera virus (BUN) is the prototype of the Bunyaviridae family. By using reverse genetics, we previously produced a recombinant virus lacking the nonstructural protein NSs (BUNdelNSs) and showed that NSs is a nonessential gene product that contributes to viral pathogenesis. Here we demonstrate that BUNdelNSs is a strong inducer of IFN-{alpha}/ß, whereas in cells infected with the wild-type counterpart expressing NSs (wild-type BUN), neither IFN nor IFN mRNA could be detected. IFN induction by BUNdelNSs correlated with activation of NF-{kappa}B and was dependent on virally produced double-stranded RNA and on the IFN transcription factor IRF-3. Furthermore, both in cultured cells and in mice lacking a functional IFN-{alpha}/ß system, BUNdelNSs replicated to wild-type BUN levels, whereas in IFN-competent systems, wild-type BUN grew more efficiently. These results suggest that BUN NSs is an IFN induction antagonist that blocks the transcriptional activation of IFN-{alpha}/ß in order to increase the virulence of Bunyamwera virus.

* Corresponding author. Mailing address: Institute of Virology, University of Glasgow, Church St., Glasgow G11 5JR, Scotland, United Kingdom. Phone: 44 141 330 4024. Fax: 44 141 337 2236. E-mail: r.elliott{at}vir.gla.ac.uk.

Journal of Virology, August 2002, p. 7949-7955, Vol. 76, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.16.7949-7955.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Eifan, S. A., Elliott, R. M. (2009). Mutational Analysis of the Bunyamwera Orthobunyavirus Nucleocapsid Protein Gene. J. Virol. 83: 11307-11317 [Abstract] [Full Text]  
  • Shi, X., Goli, J., Clark, G., Brauburger, K., Elliott, R. M. (2009). Functional analysis of the Bunyamwera orthobunyavirus Gc glycoprotein. J. Gen. Virol. 90: 2483-2492 [Abstract] [Full Text]  
  • Mohamed, M., McLees, A., Elliott, R. M. (2009). Viruses in the Anopheles A, Anopheles B, and Tete Serogroups in the Orthobunyavirus Genus (Family Bunyaviridae) Do Not Encode an NSs Protein. J. Virol. 83: 7612-7618 [Abstract] [Full Text]  
  • Shi, X., Elliott, R. M. (2009). Generation and analysis of recombinant Bunyamwera orthobunyaviruses expressing V5 epitope-tagged L proteins. J. Gen. Virol. 90: 297-306 [Abstract] [Full Text]  
  • Bartlett, E. J., Cruz, A.-M., Esker, J., Castano, A., Schomacker, H., Surman, S. R., Hennessey, M., Boonyaratanakornkit, J., Pickles, R. J., Collins, P. L., Murphy, B. R., Schmidt, A. C. (2008). Human Parainfluenza Virus Type 1 C Proteins Are Nonessential Proteins That Inhibit the Host Interferon and Apoptotic Responses and Are Required for Efficient Replication in Nonhuman Primates. J. Virol. 82: 8965-8977 [Abstract] [Full Text]  
  • Alff, P. J., Sen, N., Gorbunova, E., Gavrilovskaya, I. N., Mackow, E. R. (2008). The NY-1 Hantavirus Gn Cytoplasmic Tail Coprecipitates TRAF3 and Inhibits Cellular Interferon Responses by Disrupting TBK1-TRAF3 Complex Formation. J. Virol. 82: 9115-9122 [Abstract] [Full Text]  
  • Aguilar, P. V., Leung, L. W., Wang, E., Weaver, S. C., Basler, C. F. (2008). A Five-Amino-Acid Deletion of the Eastern Equine Encephalitis Virus Capsid Protein Attenuates Replication in Mammalian Systems but Not in Mosquito Cells. J. Virol. 82: 6972-6983 [Abstract] [Full Text]  
  • Randall, R. E., Goodbourn, S. (2008). Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures. J. Gen. Virol. 89: 1-47 [Abstract] [Full Text]  
  • Ogawa, Y., Sugiura, K., Kato, K., Tohya, Y., Akashi, H. (2007). Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I. J. Gen. Virol. 88: 3385-3390 [Abstract] [Full Text]  
  • Poenisch, M., Wille, S., Ackermann, A., Staeheli, P., Schneider, U. (2007). The X Protein of Borna Disease Virus Serves Essential Functions in the Viral Multiplication Cycle. J. Virol. 81: 7297-7299 [Abstract] [Full Text]  
  • Blakqori, G., Delhaye, S., Habjan, M., Blair, C. D., Sanchez-Vargas, I., Olson, K. E., Attarzadeh-Yazdi, G., Fragkoudis, R., Kohl, A., Kalinke, U., Weiss, S., Michiels, T., Staeheli, P., Weber, F. (2007). La Crosse Bunyavirus Nonstructural Protein NSs Serves To Suppress the Type I Interferon System of Mammalian Hosts. J. Virol. 81: 4991-4999 [Abstract] [Full Text]  
  • Shabman, R. S., Morrison, T. E., Moore, C., White, L., Suthar, M. S., Hueston, L., Rulli, N., Lidbury, B., Ting, J. P.-Y., Mahalingam, S., Heise, M. T. (2007). Differential Induction of Type I Interferon Responses in Myeloid Dendritic Cells by Mosquito and Mammalian-Cell-Derived Alphaviruses. J. Virol. 81: 237-247 [Abstract] [Full Text]  
  • Leonard, V. H. J., Kohl, A., Hart, T. J., Elliott, R. M. (2006). Interaction of Bunyamwera Orthobunyavirus NSs Protein with Mediator Protein MED8: a Mechanism for Inhibiting the Interferon Response. J. Virol. 80: 9667-9675 [Abstract] [Full Text]  
  • Alff, P. J., Gavrilovskaya, I. N., Gorbunova, E., Endriss, K., Chong, Y., Geimonen, E., Sen, N., Reich, N. C., Mackow, E. R. (2006). The Pathogenic NY-1 Hantavirus G1 Cytoplasmic Tail Inhibits RIG-I- and TBK-1-Directed Interferon Responses. J. Virol. 80: 9676-9686 [Abstract] [Full Text]  
  • Shi, X., Kohl, A., Leonard, V. H. J., Li, P., McLees, A., Elliott, R. M. (2006). Requirement of the N-Terminal Region of Orthobunyavirus Nonstructural Protein NSm for Virus Assembly and Morphogenesis.. J. Virol. 80: 8089-8099 [Abstract] [Full Text]  
  • Rezaee, S. A. R., Cunningham, C., Davison, A. J., Blackbourn, D. J. (2006). Kaposi's sarcoma-associated herpesvirus immune modulation: an overview. J. Gen. Virol. 87: 1781-1804 [Abstract] [Full Text]  
  • Delhaye, S., Paul, S., Blakqori, G., Minet, M., Weber, F., Staeheli, P., Michiels, T. (2006). Neurons produce type I interferon during viral encephalitis. Proc. Natl. Acad. Sci. USA 103: 7835-7840 [Abstract] [Full Text]  
  • Peng, T., Kotla, S., Bumgarner, R. E., Gustin, K. E. (2006). Human rhinovirus attenuates the type I interferon response by disrupting activation of interferon regulatory factor 3.. J. Virol. 80: 5021-5031 [Abstract] [Full Text]  
  • Shi, X., Brauburger, K., Elliott, R. M. (2005). Role of N-Linked Glycans on Bunyamwera Virus Glycoproteins in Intracellular Trafficking, Protein Folding, and Virus Infectivity. J. Virol. 79: 13725-13734 [Abstract] [Full Text]  
  • Lowen, A. C., Elliott, R. M. (2005). Mutational Analyses of the Nonconserved Sequences in the Bunyamwera Orthobunyavirus S Segment Untranslated Regions. J. Virol. 79: 12861-12870 [Abstract] [Full Text]  
  • Kuno, G., Chang, G.-J. J. (2005). Biological Transmission of Arboviruses: Reexamination of and New Insights into Components, Mechanisms, and Unique Traits as Well as Their Evolutionary Trends. Clin. Microbiol. Rev. 18: 608-637 [Abstract] [Full Text]  
  • Blakqori, G., Weber, F. (2005). Efficient cDNA-Based Rescue of La Crosse Bunyaviruses Expressing or Lacking the Nonstructural Protein NSs. J. Virol. 79: 10420-10428 [Abstract] [Full Text]  
  • Shaw, M. L., Cardenas, W. B., Zamarin, D., Palese, P., Basler, C. F. (2005). Nuclear Localization of the Nipah Virus W Protein Allows for Inhibition of both Virus- and Toll-Like Receptor 3-Triggered Signaling Pathways. J. Virol. 79: 6078-6088 [Abstract] [Full Text]  
  • Spiegel, M., Pichlmair, A., Martinez-Sobrido, L., Cros, J., Garcia-Sastre, A., Haller, O., Weber, F. (2005). Inhibition of Beta Interferon Induction by Severe Acute Respiratory Syndrome Coronavirus Suggests a Two-Step Model for Activation of Interferon Regulatory Factor 3. J. Virol. 79: 2079-2086 [Abstract] [Full Text]  
  • Soldan, S. S., Plassmeyer, M. L., Matukonis, M. K., Gonzalez-Scarano, F. (2005). La Crosse Virus Nonstructural Protein NSs Counteracts the Effects of Short Interfering RNA. J. Virol. 79: 234-244 [Abstract] [Full Text]  
  • Hagmaier, K., Gelderblom, H. R., Kochs, G. (2004). Functional comparison of the two gene products of Thogoto virus segment 6. J. Gen. Virol. 85: 3699-3708 [Abstract] [Full Text]  
  • Kohl, A., Dunn, E. F., Lowen, A. C., Elliott, R. M. (2004). Complementarity, sequence and structural elements within the 3' and 5' non-coding regions of the Bunyamwera orthobunyavirus S segment determine promoter strength. J. Gen. Virol. 85: 3269-3278 [Abstract] [Full Text]  
  • Billecocq, A., Spiegel, M., Vialat, P., Kohl, A., Weber, F., Bouloy, M., Haller, O. (2004). NSs Protein of Rift Valley Fever Virus Blocks Interferon Production by Inhibiting Host Gene Transcription. J. Virol. 78: 9798-9806 [Abstract] [Full Text]  
  • Thomas, D., Blakqori, G., Wagner, V., Banholzer, M., Kessler, N., Elliott, R. M., Haller, O., Weber, F. (2004). Inhibition of RNA Polymerase II Phosphorylation by a Viral Interferon Antagonist. J. Biol. Chem. 279: 31471-31477 [Abstract] [Full Text]  
  • Shaw, M. L., Garcia-Sastre, A., Palese, P., Basler, C. F. (2004). Nipah Virus V and W Proteins Have a Common STAT1-Binding Domain yet Inhibit STAT1 Activation from the Cytoplasmic and Nuclear Compartments, Respectively. J. Virol. 78: 5633-5641 [Abstract] [Full Text]  
  • Kohl, A., Hart, T. J., Noonan, C., Royall, E., Roberts, L. O., Elliott, R. M. (2004). A Bunyamwera Virus Minireplicon System in Mosquito Cells. J. Virol. 78: 5679-5685 [Abstract] [Full Text]  
  • Barr, J. N., Wertz, G. W. (2004). Bunyamwera Bunyavirus RNA Synthesis Requires Cooperation of 3'- and 5'-Terminal Sequences. J. Virol. 78: 1129-1138 [Abstract] [Full Text]  
  • Colon-Ramos, D. A., Irusta, P. M., Gan, E. C., Olson, M. R., Song, J., Morimoto, R. I., Elliott, R. M., Lombard, M., Hollingsworth, R., Hardwick, J. M., Smith, G. K., Kornbluth, S. (2003). Inhibition of Translation and Induction of Apoptosis by Bunyaviral Nonstructural Proteins Bearing Sequence Similarity to Reaper. Mol. Biol. Cell 14: 4162-4172 [Abstract] [Full Text]  
  • Valarcher, J.-F., Furze, J., Wyld, S., Cook, R., Conzelmann, K.-K., Taylor, G. (2003). Role of Alpha/Beta Interferons in the Attenuation and Immunogenicity of Recombinant Bovine Respiratory Syncytial Viruses Lacking NS Proteins. J. Virol. 77: 8426-8439 [Abstract] [Full Text]  
  • Basler, C. F., Mikulasova, A., Martinez-Sobrido, L., Paragas, J., Muhlberger, E., Bray, M., Klenk, H.-D., Palese, P., Garcia-Sastre, A. (2003). The Ebola Virus VP35 Protein Inhibits Activation of Interferon Regulatory Factor 3. J. Virol. 77: 7945-7956 [Abstract] [Full Text]  
  • Kohl, A., Clayton, R. F., Weber, F., Bridgen, A., Randall, R. E., Elliott, R. M. (2003). Bunyamwera Virus Nonstructural Protein NSs Counteracts Interferon Regulatory Factor 3-Mediated Induction of Early Cell Death. J. Virol. 77: 7999-8008 [Abstract] [Full Text]  
  • Streitenfeld, H., Boyd, A., Fazakerley, J. K., Bridgen, A., Elliott, R. M., Weber, F. (2003). Activation of PKR by Bunyamwera Virus Is Independent of the Viral Interferon Antagonist NSs. J. Virol. 77: 5507-5511 [Abstract] [Full Text]  
  • Blakqori, G., Kochs, G., Haller, O., Weber, F. (2003). Functional L polymerase of La Crosse virus allows in vivo reconstitution of recombinant nucleocapsids. J. Gen. Virol. 84: 1207-1214 [Abstract] [Full Text]  
  • Hagmaier, K., Jennings, S., Buse, J., Weber, F., Kochs, G. (2003). Novel Gene Product of Thogoto Virus Segment 6 Codes for an Interferon Antagonist. J. Virol. 77: 2747-2752 [Abstract] [Full Text]  
  • Young, D. F., Andrejeva, L., Livingstone, A., Goodbourn, S., Lamb, R. A., Collins, P. L., Elliott, R. M., Randall, R. E. (2003). Virus Replication in Engineered Human Cells That Do Not Respond to Interferons. J. Virol. 77: 2174-2181 [Abstract] [Full Text]  
  • Botten, J., Mirowsky, K., Kusewitt, D., Ye, C., Gottlieb, K., Prescott, J., Hjelle, B. (2002). Persistent Sin Nombre Virus Infection in the Deer Mouse (Peromyscus maniculatus) Model: Sites of Replication and Strand-Specific Expression. J. Virol. 77: 1540-1550 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»