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Journal of Virology, August 2002, p. 7883-7889, Vol. 76, No. 15
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.15.7883-7889.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Chimeric Human T-Cell Lymphotropic Virus Type 1 with the Envelope Glycoprotein of Moloney Murine Leukemia Virus Is Infectious for Murine Cells

Frédéric Delebecque,1 Karin Pramberger,1 Marie-Christine Prévost,2 Michel Brahic,1 and Frédéric Tangy1*

Unité des Virus Lents, URA 1930 CNRS,1 Plateforme de Microscopie Electronique, Institut Pasteur, Paris, France2

Received 20 February 2002/ Accepted 22 April 2002

We constructed a chimeric human T-cell lymphotropic virus type 1 (HTLV-1) provirus in which the original envelope precursor sequence was replaced by that of ecotropic Moloney murine leukemia virus (Mo-MuLV). Chimeric particles produced by transient transfection of this chimeric provirus were infectious for murine cells, such as NIH 3T3 fibroblasts, lymphoid EL4 cells, and primary CD4+ T lymphocytes, whereas HTLV-1 particles were not. The infectivity of chimeric particles increased 10 times when the R peptide located at the carboxy terminus of the MuLV envelope glycoprotein was deleted. Primary murine CD4+ T lymphocytes, infected by the {Delta}R chimeric virus, released particles that could spread the infection to other naive murine lymphoid cells. This chimeric virus, with the Mo-MuLV envelope glycoprotein and the replication characteristics of HTLV-1, should be useful in studying the pathogenesis of HTLV-1 in a mouse model.

* Corresponding author. Mailing address: Unité des Virus Lents, Institut Pasteur, 28 rue du Dr. Roux, 75274 Paris Cedex 15, France. Phone: (33) 1 45 68 87 73. Fax: (33) 1 40 61 31 67. E-mail: ftangy{at}pasteur.fr.

Journal of Virology, August 2002, p. 7883-7889, Vol. 76, No. 15
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.15.7883-7889.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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