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Journal of Virology, July 2002, p. 7343-7348, Vol. 76, No. 14
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.14.7343-7348.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PG13 Packaging Cells Produce Recombinant Retroviruses Carrying a Diphtheria Toxin Mutant Which Kills Cancer Cells

Jian Qiao and Manuel Caruso*

Le Centre de Recherche en Cancérologie de l'Université Laval, L'Hôtel Dieu de Québec, Centre Hospitalier Universitaire de Québec, Québec G1R 2J6, Canada

Received 1 February 2002/ Accepted 2 April 2002

The development of suicide gene therapy with gene products that are directly toxic to cells, such as the A subunit of diphtheria toxin (DT-A), has been hampered by the difficulty of engineering recombinant viruses. DT-A is a strong inhibitor of protein synthesis that acts by ADP-ribosylating elongation factor 2, and a low level of DT-A expression in virus producer cells prevents the production of recombinant virus. We analyzed here the natural resistance of packaging cells to DT-A toxicity, and we report that PG13 and PA317 packaging cell lines are resistant to H21G, a DT-A mutant. PG13 cells produce recombinant H21G virus that efficiently kills a variety of human tumor cells. Our finding indicates that PG13 packaging cells provide a new potential for the development of DT-A-based suicide gene therapy.

* Corresponding author. Mailing address: Le Centre de Recherche en Cancérologie de l'Université Laval, L'Hôtel Dieu de Québec, Centre Hospitalier Universitaire de Québec, 9 rue Mac Mahon, Québec G1R 2J6, Canada. Phone: (418) 525-4444, ext. 5537. Fax: (418) 691-5439. E-mail: manuel.caruso{at}crhdq.ulaval.ca.

Journal of Virology, July 2002, p. 7343-7348, Vol. 76, No. 14
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.14.7343-7348.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakayama, K., Pergolizzi, R. G., Crystal, R. G. (2005). Gene Transfer-Mediated Pre-mRNA Segmental Trans-splicing As a Strategy to Deliver Intracellular Toxins for Cancer Therapy. Cancer Res. 65: 254-263 [Abstract] [Full Text]  


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