This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Trgovcich, J.
Right arrow Articles by Roizman, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trgovcich, J.
Right arrow Articles by Roizman, B.

 Previous Article  |  Next Article 

Journal of Virology, July 2002, p. 6974-6986, Vol. 76, No. 14
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.14.6974-6986.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cell Surface Major Histocompatibility Complex Class II Proteins Are Regulated by the Products of the {gamma}134.5 and UL41 Genes of Herpes Simplex Virus 1

Joanne Trgovcich,1 David Johnson,2 and Bernard Roizman1*

The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois 60637,1 Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 972012

Received 25 January 2002/ Accepted 18 April 2002

Modulation of host immune responses has emerged as a common strategy employed by herpesviruses both to establish life-long infections and to affect recovery from infection. Herpes simplex virus 1 (HSV-1) blocks the major histocompatibility complex (MHC) class I antigen presentation pathway by inhibiting peptide transport into the endoplasmic reticulum. The interaction of viral gene products with the MHC class II pathway, however, has not been thoroughly investigated, although CD4+ T cells play an important role in human recovery from infection. We have investigated the stability, distribution, and state of MHC class II proteins in glioblastoma cells infected with wild-type HSV-1 or mutants lacking specific genes. We report the following findings. (i) Wild-type virus infection caused a decrease in the accumulation of class II protein on the surface of cells and a decrease in the endocytosis of lucifer yellow or dextran conjugated to fluorescein isothiocyanate but no decrease in the total amount of MHC class II proteins relative to the levels seen in mock-infected cells. (ii) Although the total amount of MHC class II protein remained unchanged, the amounts of cell surface MHC class II proteins were higher in cells infected with the UL41-negative mutant, which lacks the virion host shutoff protein, and especially high in cells infected with the {gamma}134.5-negative mutant. We conclude that infected cells attempt to respond to infection by increased acquisition of antigens and transport of MHC class II proteins to the cell surface and that these responses are blocked in part by the virion host shutoff protein encoded by the UL41 gene and in large measure by the direct or indirect action of the infected cell protein 34.5, the product of the {gamma}134.5 gene.

* Corresponding author. Mailing address: The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, 910 E. 58th St., Chicago, IL 60637. Phone: (773) 702-1898. Fax: (773) 702-1631. E-mail: bernard{at}cummings.uchicago.edu.

Journal of Virology, July 2002, p. 6974-6986, Vol. 76, No. 14
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.14.6974-6986.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Leib, D. A., Alexander, D. E., Cox, D., Yin, J., Ferguson, T. A. (2009). Interaction of ICP34.5 with Beclin 1 Modulates Herpes Simplex Virus Type 1 Pathogenesis through Control of CD4+ T-Cell Responses. J. Virol. 83: 12164-12171 [Abstract] [Full Text]  
  • Verpooten, D., Feng, Z., Valyi-Nagy, T., Ma, Y., Jin, H., Yan, Z., Zhang, C., Cao, Y., He, B. (2009). Dephosphorylation of eIF2{alpha} Mediated by the {gamma}134.5 Protein of Herpes Simplex Virus 1 Facilitates Viral Neuroinvasion. J. Virol. 83: 12626-12630 [Abstract] [Full Text]  
  • Jin, H., Ma, Y., Prabhakar, B. S., Feng, Z., Valyi-Nagy, T., Yan, Z., Verpooten, D., Zhang, C., Cao, Y., He, B. (2009). The {gamma}134.5 Protein of Herpes Simplex Virus 1 Is Required To Interfere with Dendritic Cell Maturation during Productive Infection. J. Virol. 83: 4984-4994 [Abstract] [Full Text]  
  • Verpooten, D., Ma, Y., Hou, S., Yan, Z., He, B. (2009). Control of TANK-binding Kinase 1-mediated Signaling by the {gamma}134.5 Protein of Herpes Simplex Virus 1. J. Biol. Chem. 284: 1097-1105 [Abstract] [Full Text]  
  • Sarma, N., Agarwal, D., Shiflett, L. A., Read, G. S. (2008). Small Interfering RNAs That Deplete the Cellular Translation Factor eIF4H Impede mRNA Degradation by the Virion Host Shutoff Protein of Herpes Simplex Virus. J. Virol. 82: 6600-6609 [Abstract] [Full Text]  
  • Alexander, D. E., Ward, S. L., Mizushima, N., Levine, B., Leib, D. A. (2007). Analysis of the Role of Autophagy in Replication of Herpes Simplex Virus in Cell Culture. J. Virol. 81: 12128-12134 [Abstract] [Full Text]  
  • Wiertz, E. J., Devlin, R., Collins, H. L., Ressing, M. E. (2007). Herpesvirus Interference with Major Histocompatibility Complex Class II-Restricted T-Cell Activation. J. Virol. 81: 4389-4396 [Full Text]  
  • Pasieka, T. J., Baas, T., Carter, V. S., Proll, S. C., Katze, M. G., Leib, D. A. (2006). Functional genomic analysis of herpes simplex virus type 1 counteraction of the host innate response.. J. Virol. 80: 7600-7612 [Abstract] [Full Text]  
  • Liang, L., Roizman, B. (2006). Herpes simplex virus 1 precludes replenishment of the short-lived receptor of tumor necrosis factor alpha by virion host shutoff-dependent degradation of its mRNA.. J. Virol. 80: 7756-7759 [Abstract] [Full Text]  
  • Barzilai, A., Zivony-Elbom, I., Sarid, R., Noah, E., Frenkel, N. (2006). The Herpes Simplex Virus Type 1 vhs-UL41 Gene Secures Viral Replication by Temporarily Evading Apoptotic Cellular Response to Infection: Vhs-UL41 Activity Might Require Interactions with Elements of Cellular mRNA Degradation Machinery. J. Virol. 80: 505-513 [Abstract] [Full Text]  
  • Jing, X., He, B. (2005). Characterization of the triplet repeats in the central domain of the {gamma}134{middle dot}5 protein of herpes simplex virus 1. J. Gen. Virol. 86: 2411-2419 [Abstract] [Full Text]  
  • Feng, P., Everly, D. N. Jr., Read, G. S. (2005). mRNA Decay during Herpes Simplex Virus (HSV) Infections: Protein-Protein Interactions Involving the HSV Virion Host Shutoff Protein and Translation Factors eIF4H and eIF4A. J. Virol. 79: 9651-9664 [Abstract] [Full Text]  
  • Prechtel, A. T., Turza, N. M., Kobelt, D. J., Eisemann, J. I., Coffin, R. S., McGrath, Y., Hacker, C., Ju, X., Zenke, M., Steinkasserer, A. (2005). Infection of mature dendritic cells with herpes simplex virus type 1 dramatically reduces lymphoid chemokine-mediated migration. J. Gen. Virol. 86: 1645-1657 [Abstract] [Full Text]  
  • Strand, S. S., Leib, D. A. (2004). Role of the VP16-Binding Domain of vhs in Viral Growth, Host Shutoff Activity, and Pathogenesis. J. Virol. 78: 13562-13572 [Abstract] [Full Text]  
  • Broberg, E. K., Peltoniemi, J., Nygardas, M., Vahlberg, T., Roytta, M., Hukkanen, V. (2004). Spread and Replication of and Immune Response to {gamma}134.5-Negative Herpes Simplex Virus Type 1 Vectors in BALB/c Mice. J. Virol. 78: 13139-13152 [Abstract] [Full Text]  
  • Jing, X., Cerveny, M., Yang, K., He, B. (2004). Replication of Herpes Simplex Virus 1 Depends on the {gamma}134.5 Functions That Facilitate Virus Response to Interferon and Egress in the Different Stages of Productive Infection. J. Virol. 78: 7653-7666 [Abstract] [Full Text]  
  • Doepker, R. C., Hsu, W.-L., Saffran, H. A., Smiley, J. R. (2004). Herpes Simplex Virus Virion Host Shutoff Protein Is Stimulated by Translation Initiation Factors eIF4B and eIF4H. J. Virol. 78: 4684-4699 [Abstract] [Full Text]  
  • Smiley, J. R. (2004). Herpes Simplex Virus Virion Host Shutoff Protein: Immune Evasion Mediated by a Viral RNase?. J. Virol. 78: 1063-1068 [Full Text]  
  • Cheng, G., Yang, K., He, B. (2003). Dephosphorylation of eIF-2{alpha} Mediated by the {gamma}134.5 Protein of Herpes Simplex Virus Type 1 Is Required for Viral Response to Interferon but Is Not Sufficient for Efficient Viral Replication. J. Virol. 77: 10154-10161 [Abstract] [Full Text]  
  • Neumann, J., Eis-Hubinger, A. M., Koch, N. (2003). Herpes Simplex Virus Type 1 Targets the MHC Class II Processing Pathway for Immune Evasion. J. Immunol. 171: 3075-3083 [Abstract] [Full Text]  
  • Murphy, J. A., Duerst, R. J., Smith, T. J., Morrison, L. A. (2003). Herpes Simplex Virus Type 2 Virion Host Shutoff Protein Regulates Alpha/Beta Interferon but Not Adaptive Immune Responses during Primary Infection In Vivo. J. Virol. 77: 9337-9345 [Abstract] [Full Text]  
  • Mao, H., Rosenthal, K. S. (2003). Strain-Dependent Structural Variants of Herpes Simplex Virus Type 1 ICP34.5 Determine Viral Plaque Size, Efficiency of Glycoprotein Processing, and Viral Release and Neuroinvasive Disease Potential. J. Virol. 77: 3409-3417 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»