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Journal of Virology, July 2002, p. 6957-6965, Vol. 76, No. 14
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.14.6957-6965.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Species-Specific Receptor Recognition by a Minor-Group Human Rhinovirus (HRV): HRV Serotype 1A Distinguishes between the Murine and the Human Low-Density Lipoprotein Receptor

Manuela Reithmayer, Andrea Reischl, Luc Snyers, and Dieter Blaas*

Institute of Medical Biochemistry, University of Vienna, Vienna Biocenter (VBC), A-1030 Vienna, Austria

Received 14 December 2001/ Accepted 18 April 2002

Human rhinoviruses (HRV) of the minor receptor group use several members of the low-density lipoprotein receptor superfamily for cell entry. These proteins are evolutionarily highly conserved throughout species and are almost ubiquitously expressed. Their common building blocks, cysteine-rich ligand binding repeats about 40 amino acids in length, exhibit considerable sequence similarity. Various numbers of these repeats are present in the different receptors. We here demonstrate that HRV type 1A (HRV1A) replicates in mouse cells without adaptation. Furthermore, although closely related to HRV2, it fails to bind to the human low-density lipoprotein receptor but recognizes the murine protein, whereas HRV2 binds equally well to both homologues. This difference went unnoticed due to the presence of other receptors, such as the low-density lipoprotein receptor-related protein, which allow species-independent attachment. The species specificity of HRV1A reported here will aid in defining amino acid residues establishing the contact between the viral surface and the receptor.

* Corresponding author. Mailing address: Institute of Medical Biochemistry, University of Vienna, Vienna Biocenter (VBC), Dr. Bohr Gasse 9/3, A-1030 Vienna, Austria. Phone: 43 1 4277 61630. Fax: 43 1 4277 9616. E-mail: dieter.blaas{at}univie.ac.at.

Journal of Virology, July 2002, p. 6957-6965, Vol. 76, No. 14
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.14.6957-6965.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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