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Journal of Virology, July 2002, p. 6825-6831, Vol. 76, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.13.6825-6831.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Interleukin-1ß Expression in Human Gastric Carcinoma with Epstein-Barr Virus Infection

Ja-Mun Chong,1* Kazuya Sakuma,2 Makoto Sudo,3 Toshio Osawa,1,3 Etsuko Ohara,1 Hiroshi Uozaki,1 Junji Shibahara,1 Kenji Kuroiwa,4,5 Shin-ichi Tominaga,4 Yoshitaka Hippo,6 Hiroyuki Aburatani,6 Nobuaki Funata,7 and Masashi Fukayama1

Department of Pathology, Graduate School of Medicine,1 Department of Applied Biological Science, Graduate School of Science and Technology,5 Division of Genome Science, Research Center for Advanced Science and Technology, The University of Tokyoand,6 Department of Pathology, Tokyo Metropolitan Komagome Hospital, Tokyo,,7 Department of Surgery,2 Department of Biochemistry, Jichi Medical School,4 Tochigi, and Department of Surgery, Yamanashi Medical UniversityYamanashi,Japan3

Received 5 September 2001/ Accepted 26 March 2002

The KT tumor is a transplantable strain of a human Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC), established in severe combined immunodeficiency (SCID) mice, with which the cytokine expression of EBVaGC can be investigated without interference from the infiltrating lymphocytes. As a part of a high-density oligonucleotide array (GeneChip) analysis of EBVaGC, the interleukin-1ß (IL-1ß) gene was the only cytokine gene that showed markedly higher expression in the KT tumor cells than in two tumor strains of EBV-negative GC. The results were confirmed by Northern blotting, Western blotting, and enzyme-linked immunosorbent assay. Furthermore, we demonstrated a positive signal for IL-1ß mRNA in the carcinoma cells of a surgically resected EBVaGC, but not in EBV-negative GC, by in situ hybridization. In vitro, IL-1ß increased the cell growth of a GC cell line, TMK1. Thus, IL-1ß may act as an autocrine growth factor in EBVaGC.

* Corresponding author. Mailing address: Department of Pathology, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan. Phone: 81-3-5841-3341. Fax: 81-3-3815-8379. E-mail: jmchong-tky{at}umin.ac.jp.

Journal of Virology, July 2002, p. 6825-6831, Vol. 76, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.13.6825-6831.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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