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Journal of Virology, June 2002, p. 6370-6375, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.6370-6375.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Virus-Like Particles of a Fish Nodavirus Display a Capsid Subunit Domain Organization Different from That of Insect Nodaviruses

Liang Tang,1 Chan-Shing Lin,2 Neel K. Krishna,1 Mark Yeager,3,4 Anette Schneemann,1 and John E. Johnson1*

Department of Molecular Biology,1 Department of Cell Biology, The Scripps Research Institute,3 Division of Cardiovascular Diseases, Scripps Clinic, La Jolla, California 92037,4 Department of Marine Resources, National Sun Yat-sen University, Kaohsiung, Taiwan2

Received 18 October 2001/ Accepted 7 March 2002

The structure of recombinant virus-like particles of malabaricus grouper nervous necrosis virus (MGNNV), a fish nodavirus isolated from the grouper Epinephelus malabaricus, was determined by electron cryomicroscopy (cryoEM) and three-dimensional reconstruction at 23-Å resolution. The cryoEM structure, sequence comparison, and protein fold recognition analysis indicate that the coat protein of MGNNV has two domains resembling those of tomato bushy stunt virus and Norwalk virus, rather than the expected single-domain coat protein of insect nodaviruses. The analysis implies that residues 83 to 216 fold as a ß-sandwich which forms the inner shell of the T=3 capsid and residues 217 to 308 form the trimeric surface protrusions observed in the cryoEM map. The structural similarities between fish nodaviruses and members of the tombusvirus and calicivirus groups provide significant new data for understanding the evolution of the nodavirus family.

* Corresponding author. Mailing address: Department of Molecular Biology MB31, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-9705. Fax: (858) 784-8660. E-mail: jackj{at}scripps.edu.

Journal of Virology, June 2002, p. 6370-6375, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.6370-6375.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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