This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ahn, J. W.
Right arrow Articles by Alber, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ahn, J. W.
Right arrow Articles by Alber, D. G.

 Previous Article  |  Next Article 

Journal of Virology, June 2002, p. 6244-6256, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.6244-6256.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Gammaherpesvirus Lytic Gene Expression as Characterized by DNA Array

Joo Wook Ahn,1 Kenneth L. Powell,1 Paul Kellam,2 and Dagmar G. Alber1*

Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT,1 Wohl Virion Centre, Department of Immunology and Molecular Pathology, Windeyer Institute, University College London, London W1T 4JF, United Kingdom2

Received 2 October 2001/ Accepted 5 March 2002

Gammaherpesviruses are associated with a number of diseases including lymphomas and other malignancies. Murine gammaherpesvirus 68 (MHV-68) constitutes the most amenable animal model for this family of pathogens. However experimental characterization of gammaherpesvirus gene expression, at either the protein or RNA level, lags behind that of other, better-studied alpha- and beta-herpesviruses. We have developed a cDNA array to globally characterize MHV-68 gene expression profiles, thus providing an experimental supplement to a genome that is chiefly annotated by homology. Viral genes started to be transcribed as early as 3 h postinfection (p.i.), and this was followed by a rapid escalation of gene expression that could be seen at 5 h p.i. Individual genes showed their own transcription profiles, and most genes were still being expressed at 18 h p.i. Open reading frames (ORFs) M3 (chemokine-binding protein), 52, and M9 (capsid protein) were particularly noticeable due to their very high levels of expression. Hierarchical cluster analysis of transcription profiles revealed four main groups of genes and allowed functional predictions to be made by comparing expression profiles of uncharacterized genes to those of genes of known function. Each gene was also categorized according to kinetic class by blocking de novo protein synthesis and viral DNA replication in vitro. One gene, ORF 73, was found to be expressed with {alpha}-kinetics, 30 genes were found to be expressed with ß-kinetics, and 42 genes were found to be expressed with {gamma}-kinetics. This fundamental characterization furthers the development of this model and provides an experimental basis for continued investigation of gammaherpesvirus pathology.

* Corresponding author. Mailing address: Wolfson Institute for Biomedical Research, The Cruciform Building, University College London, Gower St., London WC1E 6BT, United Kingdom. Phone: 44 20 7679 6718. Fax: 44 20 7813 2846. E-mail: d.alber{at}ucl.ac.uk.

Journal of Virology, June 2002, p. 6244-6256, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.6244-6256.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tsao, E. H., Kellam, P., Sin, C. S. Y., Rasaiyaah, J., Griffiths, P. D., Clark, D. A. (2009). Microarray-based determination of the lytic cascade of human herpesvirus 6B. J. Gen. Virol. 90: 2581-2591 [Abstract] [Full Text]  
  • Flach, B., Steer, B., Thakur, N. N., Haas, J., Adler, H. (2009). The M10 Locus of Murine Gammaherpesvirus 68 Contributes to both the Lytic and the Latent Phases of Infection. J. Virol. 83: 8163-8172 [Abstract] [Full Text]  
  • Wu, T.-T., Park, T., Kim, H., Tran, T., Tong, L., Martinez-Guzman, D., Reyes, N., Deng, H., Sun, R. (2009). ORF30 and ORF34 Are Essential for Expression of Late Genes in Murine Gammaherpesvirus 68. J. Virol. 83: 2265-2273 [Abstract] [Full Text]  
  • Gredmark-Russ, S., Cheung, E. J., Isaacson, M. K., Ploegh, H. L., Grotenbreg, G. M. (2008). The CD8 T-Cell Response against Murine Gammaherpesvirus 68 Is Directed toward a Broad Repertoire of Epitopes from both Early and Late Antigens. J. Virol. 82: 12205-12212 [Abstract] [Full Text]  
  • Ling, P. D., Tan, J., Sewatanon, J., Peng, R. (2008). Murine Gammaherpesvirus 68 Open Reading Frame 75c Tegument Protein Induces the Degradation of PML and Is Essential for Production of Infectious Virus. J. Virol. 82: 8000-8012 [Abstract] [Full Text]  
  • Herskowitz, J. H., Siegel, A. M., Jacoby, M. A., Speck, S. H. (2008). Systematic Mutagenesis of the Murine Gammaherpesvirus 68 M2 Protein Identifies Domains Important for Chronic Infection. J. Virol. 82: 3295-3310 [Abstract] [Full Text]  
  • Forrest, J. C., Paden, C. R., Allen, R. D. III, Collins, J., Speck, S. H. (2007). ORF73-Null Murine Gammaherpesvirus 68 Reveals Roles for mLANA and p53 in Virus Replication. J. Virol. 81: 11957-11971 [Abstract] [Full Text]  
  • Bortz, E., Wang, L., Jia, Q., Wu, T.-T., Whitelegge, J. P., Deng, H., Zhou, Z. H., Sun, R. (2007). Murine Gammaherpesvirus 68 ORF52 Encodes a Tegument Protein Required for Virion Morphogenesis in the Cytoplasm. J. Virol. 81: 10137-10150 [Abstract] [Full Text]  
  • Wong, E., Wu, T.-T., Reyes, N., Deng, H., Sun, R. (2007). Murine Gammaherpesvirus 68 Open Reading Frame 24 Is Required for Late Gene Expression after DNA Replication. J. Virol. 81: 6761-6764 [Abstract] [Full Text]  
  • Arumugaswami, V., Wu, T.-T., Martinez-Guzman, D., Jia, Q., Deng, H., Reyes, N., Sun, R. (2006). ORF18 Is a Transfactor That Is Essential for Late Gene Transcription of a Gammaherpesvirus. J. Virol. 80: 9730-9740 [Abstract] [Full Text]  
  • Lua, D. T., Yasuike, M., Hirono, I., Aoki, T. (2005). Transcription Program of Red Sea Bream Iridovirus as Revealed by DNA Microarrays. J. Virol. 79: 15151-15164 [Abstract] [Full Text]  
  • Barton, E. S., Lutzke, M. L., Rochford, R., Virgin, H. W. IV (2005). Alpha/Beta Interferons Regulate Murine Gammaherpesvirus Latent Gene Expression and Reactivation from Latency. J. Virol. 79: 14149-14160 [Abstract] [Full Text]  
  • Boname, J. M., May, J. S., Stevenson, P. G. (2005). Murine Gammaherpesvirus 68 Open Reading Frame 11 Encodes a Nonessential Virion Component. J. Virol. 79: 3163-3168 [Abstract] [Full Text]  
  • Simas, J. P., Marques, S., Bridgeman, A., Efstathiou, S., Adler, H. (2004). The M2 gene product of murine gammaherpesvirus 68 is required for efficient colonization of splenic follicles but is not necessary for expansion of latently infected germinal centre B cells. J. Gen. Virol. 85: 2789-2797 [Abstract] [Full Text]  
  • Obar, J. J., Crist, S. G., Gondek, D. C., Usherwood, E. J. (2004). Different Functional Capacities of Latent and Lytic Antigen-Specific CD8 T Cells in Murine Gammaherpesvirus Infection. J. Immunol. 172: 1213-1219 [Abstract] [Full Text]  
  • May, J. S., Coleman, H. M., Smillie, B., Efstathiou, S., Stevenson, P. G. (2004). Forced lytic replication impairs host colonization by a latency-deficient mutant of murine gammaherpesvirus-68. J. Gen. Virol. 85: 137-146 [Abstract] [Full Text]  
  • Bortz, E., Whitelegge, J. P., Jia, Q., Zhou, Z. H., Stewart, J. P., Wu, T.-T., Sun, R. (2003). Identification of Proteins Associated with Murine Gammaherpesvirus 68 Virions. J. Virol. 77: 13425-13432 [Abstract] [Full Text]  
  • Symensma, T. L., Martinez-Guzman, D., Jia, Q., Bortz, E., Wu, T.-T., Rudra-Ganguly, N., Cole, S., Herschman, H., Sun, R. (2003). COX-2 Induction during Murine Gammaherpesvirus 68 Infection Leads to Enhancement of Viral Gene Expression. J. Virol. 77: 12753-12763 [Abstract] [Full Text]  
  • Martinez-Guzman, D., Rickabaugh, T., Wu, T.-T., Brown, H., Cole, S., Song, M. J., Tong, L., Sun, R. (2003). Transcription Program of Murine Gammaherpesvirus 68. J. Virol. 77: 10488-10503 [Abstract] [Full Text]  
  • Nakamura, H., Lu, M., Gwack, Y., Souvlis, J., Zeichner, S. L., Jung, J. U. (2003). Global Changes in Kaposi's Sarcoma-Associated Virus Gene Expression Patterns following Expression of a Tetracycline-Inducible Rta Transactivator. J. Virol. 77: 4205-4220 [Abstract] [Full Text]  
  • Jia, Q., Sun, R. (2003). Inhibition of Gammaherpesvirus Replication by RNA Interference. J. Virol. 77: 3301-3306 [Abstract] [Full Text]  
  • Ebrahimi, B., Dutia, B. M., Roberts, K. L., Garcia-Ramirez, J. J., Dickinson, P., Stewart, J. P., Ghazal, P., Roy, D. J., Nash, A. A. (2003). Transcriptome profile of murine gammaherpesvirus-68 lytic infection. J. Gen. Virol. 84: 99-109 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»