This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weston, J.
Right arrow Articles by Todd, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weston, J.
Right arrow Articles by Todd, D.

 Previous Article  |  Next Article 

Journal of Virology, June 2002, p. 6155-6163, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.6155-6163.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of Two Aquatic Alphaviruses, Salmon Pancreas Disease Virus and Sleeping Disease Virus, by Using Genome Sequence Analysis, Monoclonal Reactivity, and Cross-Infection

Jonathan Weston,1 Stéphane Villoing,2 Michel Brémont,3 Jeanette Castric,4 Martin Pfeffer,5 Victoria Jewhurst,1 Marian McLoughlin,6 OddMagne Rødseth,2 Karen Elina Christie,2 Joseph Koumans,7 and Daniel Todd8*

Department of Veterinary Sciences, The Queen's University of Belfast,1 Department of Agriculture and Rural Development, Veterinary Sciences Division, Stormont, Belfast BT4 3SD,8 35 Cherryvalley Park, Belfast BT5 6PN, United Kingdom,6 Intervet Norbio, Bergen, Norway,2 Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas,3 Laboratoire de Pathologie des Animaux Aquatiques, AFSSA Brest, Technopole Brest-Iroise, 29280 Plouzané, France,4 Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig-Maximillians University, Munich, Germany,5 Intervet International BV, Boxmeer, The Netherlands7

Received 19 November 2001/ Accepted 19 March 2002

Cell culture isolates of salmon pancreas disease virus (SPDV) of farmed Atlantic salmon and sleeping disease virus (SDV) of rainbow trout were compared. Excluding the poly(A) tracts, the genomic nucleotide sequences of SPDV and SDV RNAs include 11,919 and 11,900 nucleotides, respectively. Phylogenetic analysis places SPDV and SDV between the New World viruses of Venezuelan equine encephalitis virus and Eastern equine encephalitis virus and the Old World viruses of Aura virus and Sindbis virus. When compared to each other, SPDV and SDV show 91.1% nucleotide sequence identity over their complete genomes, with 95 and 93.6% amino acid identities over their nonstructural and structural proteins, respectively. Notable differences between the two viruses include a 24-nucleotide insertion in the C terminus of nsP3 protein of SPDV and amino acid sequence variation at the C termini of the capsid and E1 proteins. Experimental infections of Atlantic salmon and rainbow trout with SPDV and SDV confirmed that the disease lesions induced by SPDV and SDV were similar in nature. Although infections with SPDV and SDV produced similar levels of histopathology in rainbow trout, SDV induced significantly less severe lesions in salmon than did SPDV. Virus neutralization tests performed with sera from experimentally infected salmon indicated that SPDV and SDV belonged to the same serotype; however, antigenic variation was detected among SDV and geographically different SPDV isolates by using monoclonal antibodies. Although SPDV and SDV exhibit minor biological differences, we conclude on the basis of the close genetic similarity that SPDV and SDV are closely related isolates of the same virus species for which the name Salmonid alphavirus is proposed.

* Corresponding author. Mailing address: Department of Veterinary Sciences, Department of Agriculture and Rural Development, Stoney Road, Belfast BT4 3SD, United Kingdom. Phone: 44 (0)28-90525814. Fax: 44 (0)28-90525773. E-mail: Daniel.Todd{at}dardni.gov.uk.

Journal of Virology, June 2002, p. 6155-6163, Vol. 76, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.12.6155-6163.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Galbraith, S. E., Sheahan, B. J., Atkins, G. J. (2006). Deletions in the hypervariable domain of the nsP3 gene attenuate Semliki Forest virus virulence.. J. Gen. Virol. 87: 937-947 [Abstract] [Full Text]  
  • Moriette, C., LeBerre, M., Lamoureux, A., Lai, T.-L., Bremont, M. (2006). Recovery of a recombinant salmonid alphavirus fully attenuated and protective for rainbow trout.. J. Virol. 80: 4088-4098 [Abstract] [Full Text]  
  • Moriette, C., LeBerre, M., Boscher, S. K., Castric, J., Bremont, M. (2005). Characterization and mapping of monoclonal antibodies against the Sleeping disease virus, an aquatic alphavirus. J. Gen. Virol. 86: 3119-3127 [Abstract] [Full Text]  
  • Kuno, G., Chang, G.-J. J. (2005). Biological Transmission of Arboviruses: Reexamination of and New Insights into Components, Mechanisms, and Unique Traits as Well as Their Evolutionary Trends. Clin. Microbiol. Rev. 18: 608-637 [Abstract] [Full Text]  
  • De, I., Fata-Hartley, C., Sawicki, S. G., Sawicki, D. L. (2003). Functional Analysis of nsP3 Phosphoprotein Mutants of Sindbis Virus. J. Virol. 77: 13106-13116 [Abstract] [Full Text]  
  • Vasiljeva, L., Merits, A., Golubtsov, A., Sizemskaja, V., Kaariainen, L., Ahola, T. (2003). Regulation of the Sequential Processing of Semliki Forest Virus Replicase Polyprotein. J. Biol. Chem. 278: 41636-41645 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»