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Journal of Virology, May 2002, p. 5043-5050, Vol. 76, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.10.5043-5050.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Human Cytomegalovirus Gene Products US2 and US11 Differ in Their Ability To Attack Major Histocompatibility Class I Heavy Chains in Dendritic Cells

Armin Rehm,1,2* Arne Engelsberg,1,2 Domenico Tortorella,3 Ida J. Körner,1 Insa Lehmann,1,2 Hidde L. Ploegh,3 and Uta E. Höpken2

Robert-Rössle-Klinik, Department of Hematology, Oncology and Tumorimmunology, Universitätsklinikum Charite,1 Max Delbrück Center for Molecular Medicine, Berlin, Germany,2 Department of Pathology, Harvard Medical School, Boston, Massachusetts3

Received 7 January 2002/ Accepted 10 February 2002

Human cytomegalovirus (HCMV) encodes several proteins that inhibit major histocompatibility complex (MHC) class I-dependent antigen presentation. The HCMV products US2 and US11 are each sufficient for causing the dislocation of human and murine MHC class I heavy chains from the lumen of the endoplasmic reticulum to the cytosol, where the heavy chains are readily degraded. The apparent redundancy of US2 and US11 has been probed predominantly in cultured cell lines, where differences in their specificities were shown for murine and human MHC class I locus products. Here, we expressed US11 and US2 via adenovirus vectors and show that US11 exhibits a superior ability to degrade MHC class I molecules in primary human dendritic cells. MHC class II complexes are unaffected by US2- and US11-mediated attack. We suggest that multiple HCMV-encoded immunoevasions have evolved complementary functions in response to diverse host cell types and tissues.

* Corresponding author. Mailing address: Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13125 Berlin, Germany. Phone: 49-30-9406-2695. Fax: 49-30-9406-2698. E-mail: arehm{at}mdc-berlin.de.

Journal of Virology, May 2002, p. 5043-5050, Vol. 76, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.10.5043-5050.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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