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Journal of Virology, May 2002, p. 4873-4885, Vol. 76, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.10.4873-4885.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Role of Regulatory Elements and the MAPK/ERK or p38 MAPK Pathways for Activation of Human Cytomegalovirus Gene Expression

Jiping Chen and Mark F. Stinski*

Department of Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa 52242

Received 6 November 2001/ Accepted 31 January 2002

A series of recombinant viruses with either site-specific mutations or various deletions of the early UL4 promoter of human cytomegalovirus were used to determine the roles of regulatory elements and the effects of the mitogen-activated protein kinase (MAPK) pathways. Viral gene expression was regulated by upstream cis-acting sites and by basic promoter elements that respond to the MAPK signal transduction pathways. Inhibitors of either the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway or the p38 MAPK pathway affected expression equally with either wild-type or mutant early UL4 promoters in the viral genome, indicating that the effects of the inhibitors are not exclusive for a single transcription factor. The minimal responsive element is the TATA box-containing early viral promoter.

* Corresponding author. Mailing address: Department of Microbiology, College of Medicine, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7792. Fax: (319) 335-9006. E-mail: mark-stinski{at}uiowa.edu

Journal of Virology, May 2002, p. 4873-4885, Vol. 76, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.10.4873-4885.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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