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Journal of Virology, May 2002, p. 4866-4872, Vol. 76, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.10.4866-4872.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Small Viral Membrane-Associated Protein P32 Is Involved in Bacteriophage PRD1 DNA Entry

A. Marika Grahn,1 Rimantas Daugelavicius,1,2 and Dennis H. Bamford1*

Department of Biosciences and Institute of Biotechnology, University of Helsinki, Helsinki, Finland,1 Department of Biochemistry and Biophysics, Vilnius University, Vilnius, Lithuania2

Received 15 November 2001/ Accepted 15 February 2002

The lipid-containing bacteriophage PRD1 infects a variety of gram-negative cells by injecting its linear double-stranded DNA genome into the host cell cytoplasm, while the protein capsid is left outside. The virus membrane and several structural proteins are involved in phage DNA entry. In this work we identified a new infectivity protein of PRD1. Disruption of gene XXXII resulted in a mutant phenotype defective in phage reproduction. The absence of the protein P32 did not compromise the particle assembly but led to a defect in phage DNA injection. In P32-deficient particles the phage membrane is unable to undergo a structural transformation from a spherical to a tubular form. Since P32- particles are able to increase the permeability of the host cell envelope to a degree comparable to that found with wild-type particles, we suggest that the tail-tube formation is needed to eject the DNA from the phage particle rather than to reach the host cell interior.

* Corresponding author. Mailing address: Viikki Biocenter 2, P.O. Box 56 (Viikinkaari 5), 00014-University of Helsinki, Finland. Phone: 358-9-19159100. Fax: 358-9-19159098. E-mail: dennis.bamford{at}helsinki.fi.

Journal of Virology, May 2002, p. 4866-4872, Vol. 76, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.10.4866-4872.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jaatinen, S. T., Viitanen, S. J., Bamford, D. H., Bamford, J. K. H. (2004). Integral Membrane Protein P16 of Bacteriophage PRD1 Stabilizes the Adsorption Vertex Structure. J. Virol. 78: 9790-9797 [Abstract] [Full Text]  
  • Gowen, B., Bamford, J. K. H., Bamford, D. H., Fuller, S. D. (2003). The Tailless Icosahedral Membrane Virus PRD1 Localizes the Proteins Involved in Genome Packaging and Injection at a Unique Vertex. J. Virol. 77: 7863-7871 [Abstract] [Full Text]  
  • Stromsten, N. J., Bamford, D. H., Bamford, J. K. H. (2003). The Unique Vertex of Bacterial Virus PRD1 Is Connected to the Viral Internal Membrane. J. Virol. 77: 6314-6321 [Abstract] [Full Text]  


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