This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kumaraguru, U.
Right arrow Articles by Rouse, B. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kumaraguru, U.
Right arrow Articles by Rouse, B. T.

 Previous Article  |  Next Article 

Journal of Virology, January 2002, p. 136-141, Vol. 76, No. 1
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.1.136-141.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Immunization with Chaperone-Peptide Complex Induces Low-Avidity Cytotoxic T Lymphocytes Providing Transient Protection against Herpes Simplex Virus Infection

Udayasankar Kumaraguru, Malgorzata Gierynska, Shanna Norman, Barry D. Bruce, and Barry T. Rouse*

Department of Microbiology and Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996-0845

Received 26 June 2001/ Accepted 26 September 2001

Heat shock proteins loaded with viral peptides were shown to induce a CD8+ T cell response and confer protective immunity against challenge with herpes simplex virus (HSV). The delivery system consisted of recombinant human hsp70 coupled to the peptide SSIEFARL, which is the immunodominant peptide epitope, recognized by HSV specific T cells in C57BL/6 mice. Immunization resulted in CD8+ T-cell responses, measured by peptide-specific tetramers and peptide-induced intracellular gamma interferon expression and cytotoxicity, similar to responses resulting from immunization with a recombinant vaccinia virus that expressed SSIEFARL as a minigene (VvgB) and UV-inactivated HSV. However, the durability of the hsp70-SSIEFARL response was less than that resulting from VvgB and HSV immunization and in addition the CD8+ T-cell responses in the memory phase were functionally less effective. Mice challenged soon after immunization showed excellent immunity, but by 90 days postimmunization this had waned to be significantly less than the level of immunity in both VvgB- and HSV-immunized mice.

* Corresponding author. Mailing address: M409, Walters Life Sciences Building, University of Tennessee, Knoxville, TN 37996. Phone: (865) 974-4026. Fax: (865) 974-4007. E-mail:btr{at}utk.edu.

Journal of Virology, January 2002, p. 136-141, Vol. 76, No. 1
0022-538X/01/$04.00+0     DOI: 10.1128/JVI.76.1.136-141.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Muller, W. J., Dong, L., Vilalta, A., Byrd, B., Wilhelm, K. M., McClurkan, C. L., Margalith, M., Liu, C., Kaslow, D., Sidney, J., Sette, A., Koelle, D. M. (2009). Herpes simplex virus type 2 tegument proteins contain subdominant T-cell epitopes detectable in BALB/c mice after DNA immunization and infection. J. Gen. Virol. 90: 1153-1163 [Abstract] [Full Text]  
  • Koelle, D. M., Magaret, A., McClurkan, C. L., Remington, M. L., Warren, T., Teofilovici, F., Wald, A. (2008). Phase I Dose-Escalation Study of a Monovalent Heat Shock Protein 70-Herpes Simplex Virus Type 2 (HSV-2) Peptide-Based Vaccine Designed To Prime or Boost CD8 T-Cell Responses in HSV-Naive and HSV-2-Infected Subjects. CVI 15: 773-782 [Abstract] [Full Text]  
  • Graner, M. W., Cumming, R. I., Bigner, D. D. (2007). The Heat Shock Response and Chaperones/Heat Shock Proteins in Brain Tumors: Surface Expression, Release, and Possible Immune Consequences. J. Neurosci. 27: 11214-11227 [Abstract] [Full Text]  
  • Zhang, X., Issagholian, A., Berg, E. A., Fishman, J. B., Nesburn, A. B., BenMohamed, L. (2005). Th-Cytotoxic T-Lymphocyte Chimeric Epitopes Extended by N{varepsilon}-Palmitoyl Lysines Induce Herpes Simplex Virus Type 1-Specific Effector CD8+ Tc1 Responses and Protect against Ocular Infection. J. Virol. 79: 15289-15301 [Abstract] [Full Text]  
  • Kumaraguru, U., Banerjee, K., Rouse, B. T. (2005). In vivo rescue of defective memory CD8+ T cells by cognate helper T cells. J. Leukoc. Biol. 78: 879-887 [Abstract] [Full Text]  
  • Doody, A. D. H., Kovalchin, J. T., Mihalyo, M. A., Hagymasi, A. T., Drake, C. G., Adler, A. J. (2004). Glycoprotein 96 Can Chaperone Both MHC Class I- and Class II-Restricted Epitopes for In Vivo Presentation, but Selectively Primes CD8+ T Cell Effector Function. J. Immunol. 172: 6087-6092 [Abstract] [Full Text]  
  • Kumaraguru, U., Suvas, S., Biswas, P. S., Azkur, A. K., Rouse, B. T. (2004). Concomitant Helper Response Rescues Otherwise Low Avidity CD8+ Memory CTLs to Become Efficient Effectors In Vivo. J. Immunol. 172: 3719-3724 [Abstract] [Full Text]  
  • Schirmbeck, R., Fissolo, N., Chaplin, P., Reimann, J. (2003). Enhanced Priming of Multispecific, Murine CD8+ T Cell Responses by DNA Vaccines Expressing Stress Protein-Binding Polytope Peptides. J. Immunol. 171: 1240-1246 [Abstract] [Full Text]  
  • Koelle, D. M., Corey, L. (2003). Recent Progress in Herpes Simplex Virus Immunobiology and Vaccine Research. Clin. Microbiol. Rev. 16: 96-113 [Abstract] [Full Text]  
  • Liu, B., DeFilippo, A. M., Li, Z. (2002). Overcoming Immune Tolerance to Cancer by Heat Shock Protein Vaccines. Molecular Cancer Therapeutics 1: 1147-1151 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»