This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, C.-Y.
Right arrow Articles by Tsai, C.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, C.-Y.
Right arrow Articles by Tsai, C.-H.

 Previous Article  |  Next Article 

Journal of Virology, March 2001, p. 2818-2824, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.2818-2824.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Sequences at the 3' Untranslated Region of Bamboo Mosaic Potexvirus RNA Interact with the Viral RNA-Dependent RNA Polymerase

Cheng-Yen Huang, Yih-Leh Huang, Menghsiao Meng, Yau-Heiu Hsu, and Ching-Hsiu Tsai*

Graduate Institute of Agricultural Biotechnology, National Chung Hsing University, Taichung 402, Taiwan

Received 3 October 2000/Accepted 18 December 2000

The 3' untranslated region (UTR) of bamboo mosaic potexvirus (BaMV) genomic RNA was found to fold into a series of stem-loop structures including a pseudoknot structure. These structures were demonstrated to be important for viral RNA replication and were believed to be recognized by the replicase (C.-P. Cheng and C.-H. Tsai, J. Mol. Biol. 288:555-565, 1999). Electrophoretic mobility shift and competition assays have now been used to demonstrate that the Escherichia coli-expressed RNA-dependent RNA polymerase domain (Delta 893) derived from BaMV open reading frame 1 could specifically bind to the 3' UTR of BaMV RNA. No competition was observed when bovine liver tRNAs or poly(I)(C) double-stranded homopolymers were used as competitors, and the cucumber mosaic virus 3' UTR was a less efficient competitor. Competition analysis with different regions of the BaMV 3' UTR showed that Delta 893 binds to at least two independent RNA binding sites, stem-loop D and the poly(A) tail. Footprinting analysis revealed that Delta 893 could protect the sequences at loop D containing the potexviral conserved hexamer motif and part of the stem of domain D from chemical cleavage.

* Corresponding author. Mailing address: Graduate Institute of Agricultural Biotechnology, National Chung Hsing University, Taichung 402, Taiwan. Phone: 886-4-284-0451. Fax: 886-4-286-0260. E-mail: chtsai1{at}dragon.nchu.edu.tw.

Journal of Virology, March 2001, p. 2818-2824, Vol. 75, No. 6
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.6.2818-2824.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Verchot-Lubicz, J., Ye, C.-M., Bamunusinghe, D. (2007). Molecular biology of potexviruses: recent advances. J. Gen. Virol. 88: 1643-1655 [Abstract] [Full Text]  
  • Hu, B., Pillai-Nair, N., Hemenway, C. (2007). Long-distance RNA-RNA interactions between terminal elements and the same subset of internal elements on the potato virus X genome mediate minus- and plus-strand RNA synthesis. RNA 13: 267-280 [Abstract] [Full Text]  
  • Lin, J.-W., Ding, M.-P., Hsu, Y.-H., Tsai, C.-H. (2007). Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of Bamboo mosaic virus. Nucleic Acids Res 35: 424-432 [Abstract] [Full Text]  
  • Lin, J.-W., Chiu, H.-N., Chen, I-H., Chen, T.-C., Hsu, Y.-H., Tsai, C.-H. (2005). Structural and Functional Analysis of the cis-Acting Elements Required for Plus-Strand RNA Synthesis of Bamboo Mosaic Virus. J. Virol. 79: 9046-9053 [Abstract] [Full Text]  
  • Huang, Y.-L., Hsu, Y.-H., Han, Y.-T., Meng, M. (2005). mRNA Guanylation Catalyzed by the S-Adenosylmethionine-dependent Guanylyltransferase of Bamboo Mosaic Virus. J. Biol. Chem. 280: 13153-13162 [Abstract] [Full Text]  
  • Huang, Y.-L., Han, Y.-T., Chang, Y.-T., Hsu, Y.-H., Meng, M. (2004). Critical Residues for GTP Methylation and Formation of the Covalent m7GMP-Enzyme Intermediate in the Capping Enzyme Domain of Bamboo Mosaic Virus. J. Virol. 78: 1271-1280 [Abstract] [Full Text]  
  • Rajendran, K. S., Nagy, P. D. (2003). Characterization of the RNA-Binding Domains in the Replicase Proteins of Tomato Bushy Stunt Virus. J. Virol. 77: 9244-9258 [Abstract] [Full Text]  
  • Osman, T. A. M., Buck, K. W. (2003). Identification of a Region of the Tobacco Mosaic Virus 126- and 183-Kilodalton Replication Proteins Which Binds Specifically to the Viral 3'-Terminal tRNA-Like Structure. J. Virol. 77: 8669-8675 [Abstract] [Full Text]  
  • Cheng, J.-H., Peng, C.-W., Hsu, Y.-H., Tsai, C.-H. (2002). The Synthesis of Minus-Strand RNA of Bamboo Mosaic Potexvirus Initiates from Multiple Sites within the Poly(A) Tail. J. Virol. 76: 6114-6120 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»