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Journal of Virology, March 2001, p. 2421-2434, Vol. 75, No. 5
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.5.2421-2434.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Microtubule-Independent Motility and Nuclear Targeting of Adenoviruses with Fluorescently Labeled Genomes

Jolanta B. Glotzer, Anne-Isabelle Michou, Adam Baker, Mediyha Saltik, and Matt Cotten*

Institute for Molecular Pathology, 1030 Vienna, Austria

Received 17 October 2000/Accepted 1 December 2000

A novel adenovirus system for analyzing the adenovirus entry pathway has been developed that contains green fluorescent protein bound to the encapsidated viral DNA (AdLite viruses). AdLite viruses enter host cells and accumulate around the nuclei and near the microtubule organizing centers (MTOC). In live cells, individual AdLite particles were observed trafficking both toward and away from the nucleus. Depolymerization of microtubules during infection prevented AdLite accumulation around the MTOC; however, it did not abolish perinuclear localization of AdLite particles. Furthermore, depolymerization of microtubules did not affect AdLite motility and did not affect gene expression from wild-type adenovirus and adenovirus-derived vectors. These data revealed that adenovirus intracellular motility and nuclear targeting can be supported by a mechanism that does not rely on the microtubule network.

* Corresponding author. Mailing address: Axxima Pharmaceuticals AG, Am Klopferspitz 19, 82152 Martinsried, Germany. Phone: (49) (89) 740 1650. Fax: (49) (89) 740 165 20. E-mail: cotten{at}axxima.com.

Journal of Virology, March 2001, p. 2421-2434, Vol. 75, No. 5
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.5.2421-2434.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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