This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bose, S.
Right arrow Articles by Banerjee, A. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bose, S.
Right arrow Articles by Banerjee, A. K.

 Previous Article  |  Next Article 

Journal of Virology, February 2001, p. 1984-1989, Vol. 75, No. 4
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.4.1984-1989.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Polarity of Human Parainfluenza Virus Type 3 Infection in Polarized Human Lung Epithelial A549 Cells: Role of Microfilament and Microtubule

Santanu Bose, Achut Malur, and Amiya K. Banerjee*

Department of Virology, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195

Received 15 September 2000/Accepted 16 November 2000

Human parainfluenza virus type 3 (HPIV-3) is an airborne pathogen that infects the epithelial cells of the respiratory tract. In the present study we investigated the interaction of HPIV-3 with the type II alveolar human lung polarized epithelial A549 cells. Although HPIV-3 entry and budding were bidirectional from both the apical and the basolateral domains, HPIV-3 exhibited preferential entry and release from the apical pole. While disruption of the cellular actin microfilament and microtubule by cytochalasin D and nocodazole, respectively, had no effect on virus entry, disruption of the microtubule but not the microfilament inhibited HPIV-3 release.

* Corresponding author. Mailing address: Department of Virology, The Cleveland Clinic Foundation, Lerner Research Institute, Room NC20, 9500 Euclid Ave., Cleveland, OH 44195. Phone: (216) 444-0625. Fax: (216) 444-0512. E-mail: banerja{at}ccf.org.

Journal of Virology, February 2001, p. 1984-1989, Vol. 75, No. 4
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.4.1984-1989.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Snooks, M. J., Bhat, P., Mackenzie, J., Counihan, N. A., Vaughan, N., Anderson, D. A. (2008). Vectorial Entry and Release of Hepatitis A Virus in Polarized Human Hepatocytes. J. Virol. 82: 8733-8742 [Abstract] [Full Text]  
  • Kota, S., Sabbah, A., Chang, T. H., Harnack, R., Xiang, Y., Meng, X., Bose, S. (2008). Role of Human {beta}-Defensin-2 during Tumor Necrosis Factor-{alpha}/NF-{kappa}B-mediated Innate Antiviral Response against Human Respiratory Syncytial Virus. J. Biol. Chem. 283: 22417-22429 [Abstract] [Full Text]  
  • Basu, M., Maitra, R. K., Xiang, Y., Meng, X., Banerjee, A. K., Bose, S. (2006). Inhibition of vesicular stomatitis virus infection in epithelial cells by alpha interferon-induced soluble secreted proteins. J. Gen. Virol. 87: 2653-2662 [Abstract] [Full Text]  
  • Hurley, B. P., Williams, N. L., McCormick, B. A. (2006). Involvement of phospholipase A2 in Pseudomonas aeruginosa-mediated PMN transepithelial migration. Am. J. Physiol. Lung Cell. Mol. Physiol. 290: L703-L709 [Abstract] [Full Text]  
  • Rowe, R. K., Pekosz, A. (2006). Bidirectional Virus Secretion and Nonciliated Cell Tropism following Andes Virus Infection of Primary Airway Epithelial Cell Cultures. J. Virol. 80: 1087-1097 [Abstract] [Full Text]  
  • Tseng, C.-T. K., Tseng, J., Perrone, L., Worthy, M., Popov, V., Peters, C. J. (2005). Apical Entry and Release of Severe Acute Respiratory Syndrome-Associated Coronavirus in Polarized Calu-3 Lung Epithelial Cells. J. Virol. 79: 9470-9479 [Abstract] [Full Text]  
  • Cordo, S. M., Acuna, M. C. y, Candurra, N. A. (2005). Polarized entry and release of Junin virus, a New World arenavirus. J. Gen. Virol. 86: 1475-1479 [Abstract] [Full Text]  
  • Zhang, L., Bukreyev, A., Thompson, C. I., Watson, B., Peeples, M. E., Collins, P. L., Pickles, R. J. (2005). Infection of Ciliated Cells by Human Parainfluenza Virus Type 3 in an In Vitro Model of Human Airway Epithelium. J. Virol. 79: 1113-1124 [Abstract] [Full Text]  
  • Hurley, B. P., Siccardi, D., Mrsny, R. J., McCormick, B. A. (2004). Polymorphonuclear Cell Transmigration Induced by Pseudomonas aeruginosa Requires the Eicosanoid Hepoxilin A3. J. Immunol. 173: 5712-5720 [Abstract] [Full Text]  
  • Bose, S., Basu, M., Banerjee, A. K. (2004). Role of Nucleolin in Human Parainfluenza Virus Type 3 Infection of Human Lung Epithelial Cells. J. Virol. 78: 8146-8158 [Abstract] [Full Text]  
  • Chiou, C.-T., Hu, C.-C. A., Chen, P.-H., Liao, C.-L., Lin, Y.-L., Wang, J.-J. (2003). Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein. J. Gen. Virol. 84: 2795-2805 [Abstract] [Full Text]  
  • Bose, S., Kar, N., Maitra, R., DiDonato, J. A., Banerjee, A. K. (2003). Temporal activation of NF-{kappa}B regulates an interferon-independent innate antiviral response against cytoplasmic RNA viruses. Proc. Natl. Acad. Sci. USA 100: 10890-10895 [Abstract] [Full Text]  
  • Bose, S., Mathur, M., Bates, P., Joshi, N., Banerjee, A. K. (2003). Requirement for cyclophilin A for the replication of vesicular stomatitis virus New Jersey serotype. J. Gen. Virol. 84: 1687-1699 [Abstract] [Full Text]  
  • Henrickson, K. J. (2003). Parainfluenza Viruses. Clin. Microbiol. Rev. 16: 242-264 [Abstract] [Full Text]  
  • Ros, C., Burckhardt, C. J., Kempf, C. (2002). Cytoplasmic Trafficking of Minute Virus of Mice: Low-pH Requirement, Routing to Late Endosomes, and Proteasome Interaction. J. Virol. 76: 12634-12645 [Abstract] [Full Text]  
  • Chu, J. J. H., Ng, M. L. (2002). Infection of polarized epithelial cells with flavivirus West Nile: polarized entry and egress of virus occur through the apical surface. J. Gen. Virol. 83: 2427-2435 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»