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Journal of Virology, February 2001, p. 1751-1760, Vol. 75, No. 4
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.4.1751-1760.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Different Regions of Primase Subunit p48 Control Mouse Polyomavirus and Simian Virus 40 DNA Replication In Vitro

Armin R. Kautz,1,dagger Annerose Schneider,1 Klaus Weisshart,1,Dagger Christiane Geiger,2 and Heinz-Peter Nasheuer1,*

Abteilung Biochemie, Institut für Molekulare Biotechnologie e.V., D-07745 Jena,1 and Genzentrum, Ludwig-Maximilians-Universität München, D-81377 Munich,2 Germany

Received 20 July 2000/Accepted 17 November 2000

DNA polymerase alpha -primase (pol-prim), a complex consisting of four subunits, is the major species-specific factor for mouse polyomavirus (PyV) and simian virus 40 (SV40) DNA replication. Although p48 is the most conserved subunit of pol-prim, it is required for in vitro PyV DNA replication but can inhibit cell-free SV40 DNA replication. Production of chimeric human-mouse p48 revealed that different regions of p48 are involved in supporting PyV DNA replication and inhibiting SV40 DNA replication. The N and C-terminal parts of p48 do not have species-specific functions in cell-free PyV DNA replication, but the central part (amino acids [aa] 129 to 320) controls PyV DNA replication in vitro. However, PyV T antigen physically binds to mouse, human, and chimeric pol-prim complexes independently, whether they support PyV DNA replication or not. In contrast to the PyV system, the inhibitory effects of mouse p48 on SV40 DNA replication are mediated by N- and C-terminal regions of p48. Thus, a chimeric p48 containing human aa 1 to 128, mouse aa 129 to 320, and human aa 321 to 418 is active in both PyV and SV40 DNA replication in vitro.

* Corresponding author. Mailing address: Institut für Molekulare Biotechnologie e.V., Abt. Biochemie, Beutenbergstr. 11, D-07745 Jena, Germany. Phone: 49-3641-656290. Fax: 49-3641-656288. E-mail: nasheuer{at}imb-jena.de.

dagger Present address: Institut für Biochemie, Universität Erlangen-Nürnberg, D-91054 Erlangen, Germany.

Dagger Present address: Carl Zeiss Jena GmbH, D-07745 Jena, Germany.

Journal of Virology, February 2001, p. 1751-1760, Vol. 75, No. 4
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.4.1751-1760.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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