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Journal of Virology, December 2001, p. 12402-12411, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12402-12411.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Stabilization but Not the Transcriptional Activity of Herpes Simplex Virus VP16-Induced Complexes Is Evolutionarily Conserved among HCF Family Members

Soyoung Lee1,2 and Winship Herr1,*

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724,1 and Program in Molecular and Cellular Biology, State University of New York, Stony Brook, New York 117942

Received 1 June 2001/Accepted 12 September 2001

The human herpes simplex virus (HSV) protein VP16 induces formation of a transcriptional regulatory complex with two cellular factors---the POU homeodomain transcription factor Oct-1 and the cell proliferation factor HCF-1---to activate viral immediate-early-gene transcription. Although the cellular role of Oct-1 in transcription is relatively well understood, the cellular role of HCF-1 in cell proliferation is enigmatic. HCF-1 and the related protein HCF-2 form an HCF protein family in humans that is related to a Caenorhabditis elegans homolog called CeHCF. In this study, we show that all three proteins can promote VP16-induced-complex formation, indicating that VP16 targets a highly conserved function of HCF proteins. The resulting VP16-induced complexes, however, display different transcriptional activities. In contrast to HCF-1 and CeHCF, HCF-2 fails to support VP16 activation of transcription effectively. These results suggest that, along with HCF-1, HCF-2 could have a role, albeit probably a different role, in HSV infection. CeHCF can mimic HCF-1 for both association with viral and cellular proteins and transcriptional activation, suggesting that the function(s) of HCF-1 targeted by VP16 has been highly conserved throughout metazoan evolution.

* Corresponding author. Mailing address: Cold Spring Harbor Laboratory, P.O. Box 100, Cold Spring Harbor, NY 11724. Phone: (516) 367-8401. Fax: (516) 367-8454. E-mail: herr{at}cshl.org.

Journal of Virology, December 2001, p. 12402-12411, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12402-12411.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yamauchi, Y., Kiriyama, K., Kubota, N., Kimura, H., Usukura, J., Nishiyama, Y. (2008). The UL14 Tegument Protein of Herpes Simplex Virus Type 1 Is Required for Efficient Nuclear Transport of the Alpha Transinducing Factor VP16 and Viral Capsids. J. Virol. 82: 1094-1106 [Abstract] [Full Text]  
  • Piluso, D., Bilan, P., Capone, J. P. (2002). Host Cell Factor-1 Interacts with and Antagonizes Transactivation by the Cell Cycle Regulatory Factor Miz-1. J. Biol. Chem. 277: 46799-46808 [Abstract] [Full Text]  


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