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Journal of Virology, December 2001, p. 12098-12104, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12098-12104.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Open Reading Frame III of Borna Disease Virus Encodes a Nonglycosylated Matrix Protein

Ina Kraus,1 Markus Eickmann,1 Simone Kiermayer,1 Hanno Scheffczik,1 Manuela Fluess,2 Jürgen A. Richt,2,dagger and Wolfgang Garten1,*

Institut für Virologie, Philipps-Universität Marburg, D-35037 Marburg,1 and Institut für Virologie, Justus-Liebig-Universität Giessen, D-35392 Giessen,2 Germany

Received 20 July 2001/Accepted 24 September 2001

The open reading frame III of Borna disease virus (BDV) codes for a protein with a mass of 16 kDa, named p16 or BDV-M. p16 was described as an N-glycosylated protein in several previous publications and therefore was termed gp18, although the amino acid sequence of p16 does not contain any regular consensus sequence for N glycosylation. We examined glycosylation of p16 and studied its membrane topology using antisera raised against peptides, which comprise the N and the C termini. Neither an N- nor a C-terminal peptide is cleaved from p16 during maturation. Neither deglycosylation of p16 by endoglycosidases nor binding of lectin to p16 was detectable. Introduction of typical N-glycosylation sites at the proposed sites of p16 failed in carbohydrate attachment. Flotation experiments with membranes of BDV-infected cells on density gradients revealed that p16 is not an integral membrane protein, since it can be dissociated from membranes. Our experimental data strongly suggest that p16 is a typical nonglycosylated matrix protein associated at the inner surface of the viral membrane, as is true for homologous proteins of other members of the Mononegavirales order.

* Corresponding author. Mailing address: Institut für Virologie, Robert-Koch-Str. 17, D-35037 Marburg, Germany. Phone: (49) 6421-28-65145. Fax: (49) 6421-28-68962. E-mail: garten{at}mailer.unimarburg.de.

dagger Present address: National Animal Disease Center, Ames, IA 50010.

Journal of Virology, December 2001, p. 12098-12104, Vol. 75, No. 24
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.24.12098-12104.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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