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Journal of Virology, December 2001, p. 11930-11934, Vol. 75, No. 23
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.23.11930-11934.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Vaccination with Attenuated Simian Immunodeficiency Virus by DNA Inoculation

Stephen J. Kent,^1,2,* C. Jane Dale,^1,2 Scott Preiss,² John Mills,² Daniella Campagna,² and Damian F. J. Purcell²

Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010,¹ and Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, 3078,² Australia

Received 2 July 2001/Accepted 30 August 2001

Delivering attenuated lentivirus vaccines as proviral DNA would be simple and inexpensive. Inoculation of macaques with wild-type simian immunodeficiency virus strain mac239 (SIV_mac239) DNA or SIV_mac239 DNA containing a single deletion in the 3' nef-long terminal repeat overlap region (nef/LTR) led to sustained SIV infections and AIDS. Injection of SIV_mac239 DNA containing identical deletions in both the 5' LTR and 3' nef/LTR resulted in attenuated SIV infections and substantial protection against subsequent mucosal SIV_mac251 challenge.

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^* Corresponding author. Mailing address: Dept. of Microbiology and Immunology, University of Melbourne, Parkville, Vic. 3010, Australia. Phone: 61383449939. Fax: 61383443846. E-mail: skent{at}unimelb.edu.au.

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Journal of Virology, December 2001, p. 11930-11934, Vol. 75, No. 23
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.23.11930-11934.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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