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Journal of Virology, November 2001, p. 10991-11001, Vol. 75, No. 22
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.22.10991-11001.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Multiple Effects of Codon Usage Optimization on Expression and Immunogenicity of DNA Candidate Vaccines Encoding the Human Immunodeficiency Virus Type 1 Gag Protein

Ludwig Deml,1 Alexandra Bojak,1 Stephanie Steck,1 Marcus Graf,1 Jens Wild,1 Reinhold Schirmbeck,2 Hans Wolf,1 and Ralf Wagner1,*

Institute of Medical Microbiology, University of Regensburg, 93053 Regensburg,1 and Institute of Medical Microbiology and Immunology, University of Ulm, 89069 Ulm,2 Germany

Received 18 April 2001/Accepted 7 August 2001

We have analyzed the influence of codon usage modifications on the expression levels and immunogenicity of DNA vaccines, encoding the human immunodeficiency virus type 1 (HIV-1) group-specific antigen (Gag). In the presence of Rev, an expression vector containing the wild-type (wt) gag gene flanked by essential cis-acting sites such as the 5'-untranslated region and 3'-Rev response element supported substantial Gag protein expression and secretion in human H1299 and monkey COS-7 cells. However, only weak Gag production was observed from the murine muscle cell line C2C12. In contrast, optimization of the Gag coding sequence to that of highly expressed mammalian genes (syngag) resulted in an obvious increase in the G+C content and a Rev-independent expression and secretion of Gag in all tested mammalian cell lines, including murine C2C12 muscle cells. Mice immunized intramuscularly with the syngag plasmid showed Th1-driven humoral and cellular responses that were substantially higher than those obtained after injection of the Rev-dependent wild-type (wt) gag vector system. In contrast, intradermal immunization of both wt gag and syngag vector systems with the particle gun induced a Th2-biased antibody response and no cytotoxic T lymphocytes. Deletion analysis demonstrated that the CpG motifs generated within syngag by codon optimization do not contribute significantly to the high immunogenicity of the syngag plasmid. Moreover, low doses of coadministered stimulatory phosphorothioate oligodeoxynucleotides (ODNs) had only a weak effect on antibody production, whereas at higher doses immunostimulatory and nonstimulatory ODNs showed a dose-dependent suppression of humoral responses. These results suggest that increased Gag expression, rather than modulation of CpG-driven vector immunity, is responsible for the enhanced immunogenicity of the syngag DNA vaccine.

* Corresponding author. Mailing address: Institute for Medical Microbiology, Klinikum Regensburg, Franz-Josef-Strauss Allee 11, 93053 Regensburg, Germany. Phone: 49 (0) 941-944-6452. Fax: 49 (0) 941-944-6402. E-mail: ralf.wagner{at}klinik.uni-regensburg.de.

Journal of Virology, November 2001, p. 10991-11001, Vol. 75, No. 22
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.22.10991-11001.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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