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Journal of Virology, November 2001, p. 10161-10169, Vol. 75, No. 21
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.21.10161-10169.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Host RNA Polymerase Requirements for Transcription of the Human Hepatitis Delta Virus Genome

Gloria Moraleda and John Taylor*

Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111-2497

Received 16 July 2001/Accepted 26 July 2001

Replication of the genome of hepatitis delta virus (HDV) requires RNA-directed RNA synthesis using a host polymerase(s). This manuscript reviews the relevant published evidence. It also provides two new studies, both of which made use of transiently transfected Huh7 cells undergoing HDV RNA-directed RNA synthesis. For the first study, RNA transcription inhibitors were added to the transfected cells for periods of 1 to 2 days, after which assays of the effects on the accumulation of processed unit-length genomic HDV RNA were performed. For the second study, nuclei were isolated at 6 days after transfection, and then in vitro runoff transcription was used to assay the effects of RNA transcription inhibitors. Overall, the data support the interpretation that HDV transcription does not require host polymerase I or III (pol I or III) but at least primarily involves an enzyme resembling pol II.

* Corresponding author. Mailing address: Fox Chase Cancer Center, 7701 Burholme Ave., Philadelphia, PA 19111-2497. Phone: (215) 728-2436. Fax: (215) 728-3105. E-mail: JM_Taylor{at}FCCC.edu.

Journal of Virology, November 2001, p. 10161-10169, Vol. 75, No. 21
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.21.10161-10169.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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