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Journal of Virology, January 2001, p. 988-995, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.988-995.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Characterization of Bombyx mori Cytoplasmic Polyhedrosis Virus Genome Segment 4

Keiko Ikeda,1 Sumiharu Nagaoka,1 Stefan Winkler,2 Kumiko Kotani,1 Hiroaki Yagi,3 Kae Nakanishi,3 Shigetoshi Miyajima,3 Jun Kobayashi,3 and Hajime Mori1,*

Department of Applied Biology, Kyoto Institute of Technology, Kyoto 606-8585,1 and Department of Chemistry for Materials, Mie University, Mie 514-8507,3 Japan, and Department of Chemical Engineering, Tufts University, Medford, Massachusetts 021552

Received 21 August 2000/Accepted 12 October 2000

The complete nucleotide sequence of the genome segment 4 (S4) of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) was determined. The 3,259-nucleotide sequence contains a single long open reading frame which spans nucleotides 14 to 3187 and which is predicted to encode a protein with a molecular mass of about 130 kDa. Western blot analysis showed that S4 encodes BmCPV protein VP3, which is one of the outer components of the BmCPV virion. Sequence analysis of the deduced amino acid sequence of BmCPV VP3 revealed possible sequence homology with proteins from rice ragged stunt virus (RRSV) S2, Nilaparvata lugens reovirus S4, and Fiji disease fijivirus S4. This may suggest that plant reoviruses originated from insect viruses and that RRSV emerged more recently than other plant reoviruses. A chimeric protein consisting of BmCPV VP3 and green fluorescent protein (GFP) was constructed and expressed with BmCPV polyhedrin using a baculovirus expression vector. The VP3-GFP chimera was incorporated into BmCPV polyhedra and released under alkaline conditions. The results indicate that specific interactions occur between BmCPV polyhedrin and VP3 which might facilitate BmCPV virion occlusion into the polyhedra.

* Corresponding author. Mailing address: Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan. Phone: 81-75-724-7776. Fax: 81-75-724-7760. E-mail: hmori{at}ipc.kit.ac.jp.

Journal of Virology, January 2001, p. 988-995, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.988-995.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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