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Journal of Virology, January 2001, p. 1031-1038, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.1031-1038.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Adenovirus Protein V Induces Redistribution of Nucleolin and B23 from Nucleolus to Cytoplasm

David A. Matthews*

Molecular Medicine Unit, University of Leeds, St. James's University Hospital, Leeds LS9 7TF, United Kingdom

Received 15 August 2000/Accepted 21 October 2000

Adenovirus infection inhibits synthesis and processing of rRNA and redistributes nucleolar antigens. Adenovirus protein V associates with nucleoli in infected cells. This study delineates regions of protein V independently capable of nucleolar targeting. Also, evidence is presented that protein V has the unique property of relocating nucleolin and B23 to the cytoplasm when transiently expressed on its own in uninfected cells. Point mutation analysis indicates a role for the C terminus of protein V in the redirection of nucleolin and B23 to the cytoplasm. This is the first time an adenovirus protein has been shown to have a direct effect on nucleolar antigens in isolation from viral infection. Moreover, adenovirus protein V is the first protein demonstrated to be capable of redirecting nucleolin and B23 to the cytoplasm.

* Mailing address: Molecular Medicine Unit, University of Leeds, Clinical Sciences Building, St. James's University Hospital, Beckett St., Leeds LS9 7TF, United Kingdom. Phone: 44 113 206 6328. Fax: 44 113 244 4475. E-mail: meddam{at}stjames.leeds.ac.uk.

Journal of Virology, January 2001, p. 1031-1038, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.1031-1038.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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