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Journal of Virology, January 2001, p. 1004-1012, Vol. 75, No. 2
Laboratory of Virology, Wageningen
University, Wageningen,1 and Department
of Infectious Diseases and Immunology, Utrecht University,
Utrecht,2 The Netherlands
Received 21 July 2000/Accepted 19 October 2000
The glycoprotein precursor (G1/G2) gene of tomato spotted wilt
virus (TSWV) was expressed in BHK cells using the Semliki Forest virus
expression system. The results reveal that in this cell system, the
precursor is efficiently cleaved and the resulting G1 and G2
glycoproteins are transported from the endoplasmic reticulum (ER) to
the Golgi complex, where they are retained, a process that could be
blocked by tunicamycin. Expression of G2 alone resulted in transport to
and retention in the Golgi complex, albeit less efficient, suggesting
that G2 contains a Golgi retention signal. G1 alone was retained in the
ER, irrespective of whether it contained the precursor's signal
sequence or its own N-terminal hydrophobic sequence. Coexpression of G1
and G2 from separate gene constructs resulted in rescue of efficient G1
transport, as the proteins coaccumulated in the Golgi complex,
indicating that their interaction is essential for proper targeting to
this organelle. The results demonstrate that transport and targeting of
the plant TSWV glycoproteins in mammalian BHK cells are strikingly
similar to those of animal-infecting bunyavirus glycoproteins in
mammalian cells. The observations are likely to reflect the dual
tropism of TSWV, which replicates both in its plant host and in its
animal (thrips) vector.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.2.1004-1012.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Tomato Spotted Wilt Virus Glycoproteins Exhibit
Trafficking and Localization Signals That Are Functional in
Mammalian Cells
*
Corresponding author. Mailing address: Laboratory of
Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands. Phone: 31-317-483090. Fax: 31-317-484820. E-mail: Rob.Goldbach{at}medew.viro.wau.nl.
Journal of Virology, January 2001, p. 1004-1012, Vol. 75, No. 2
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.2.1004-1012.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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