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Journal of Virology, October 2001, p. 9470-9482, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9470-9482.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

A Cellular J-Domain Protein Modulates Polyprotein Processing and Cytopathogenicity of a Pestivirus

G. Rinck,1,dagger C. Birghan,1 T. Harada,1,Dagger G. Meyers,2 H.-J. Thiel,1 and N. Tautz1,*

Institut für Virologie (FB Veterinärmedizin), Justus-Liebig-Universität Giessen, D-35392 Giessen,1 and Federal Research Center for Virus Diseases of Animals, D-72076 Tübingen,2 Germany

Received 5 April 2001/Accepted 21 June 2001

Pestiviruses are positive-strand RNA viruses closely related to human hepatitis C virus. Gene expression of these viruses occurs via translation of a polyprotein, which is further processed by cellular and viral proteases. Here we report the formation of a stable complex between an as-yet-undescribed cellular J-domain protein, a member of the DnaJ-chaperone family, and pestiviral nonstructural protein NS2. Accordingly, we termed the cellular protein Jiv, for J-domain protein interacting with viral protein. Jiv has the potential to induce in trans one specific processing step in the viral polyprotein, namely, cleavage of NS2-3. Efficient generation of its cleavage product NS3 has previously been shown to be obligatory for the cytopathogenicity of the pestiviruses. Regulated expression of Jiv in cells infected with noncytopathogenic bovine viral diarrhea virus disclosed a direct correlation between the intracellular level of Jiv, the extent of NS2-3 cleavage, and pestiviral cytopathogenicity.

* Corresponding author. Mailing address: Institut für Virologie (FB Veterinärmedizin), Justus-Liebig-Universität Giessen, Frankfurter Strasse 107, D-35392 Giessen, Germany. Phone: 49 (641) 99-38375. Fax: 49 (641) 99-38359. E-mail: Norbert.Tautz{at}vetmed.uni-giessen.de.

dagger Present address: Virco UK, Ltd., Cambridge, United Kingdom.

Dagger Present address: Chiron Corp., I-53100 Siena, Italy.

Journal of Virology, October 2001, p. 9470-9482, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9470-9482.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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